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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 10, 2011 |
| Title |
Transcript level in F9 teratocarcinoma WT and RARalpha knockout in presence and absence of all-trans retinoic acid |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Retinoic acid receptors (RARs) α, β and γ are key regulators of embryonic development. Hematopoietic differentiation is regulated by RARα, and several types of leukemia show aberrant RARα activity. We demonstrate that RARα plays an important role in cellular memory and imprinting by regulating the CpG methylation status of specific promoter regions.
We used microarrays to identify genes, which display differential expression in F9 RARalpha knockout (RARaKO) cells relative to F9 wt cells.
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| Overall design |
F9 teratocarcinoma cells (WT and RARaKO) were treated for 8 hours with either vehicle (EtOH) or all-trans Retinoic Acid (atRA) in the presence of the protein synthesis inhibitor Cycloheximide
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| Contributor(s) |
Laursen KB, Gudas LJ |
| Citation(s) |
21911359 |
| Submission date |
Aug 09, 2011 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Kristian Bruun Laursen |
| E-mail(s) |
krl2004@med.cornell.edu
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| Organization name |
Weill Cornell Medical College
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| Department |
Pharmacology
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| Lab |
Lorraine Gudas
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| Street address |
1300 York Avenue
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| City |
New York |
| ZIP/Postal code |
NY 10065 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| GSM775294 |
atRA WT, set 2 |
| GSM775295 |
EtOH, RARaKO, set 3 |
| GSM775296 |
atRA RARaKO, set 3 |
| GSM775297 |
EtOH, WT, set 3 |
| GSM775298 |
atRA WT, set 3 |
| GSM775299 |
EtOH, RARaKO, set 4 |
| GSM775300 |
atRA RARaKO, set 4 |
| GSM775301 |
EtOH, WT, set 4 |
| GSM775302 |
atRA WT, set 4 |
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| Relations |
| BioProject |
PRJNA145853 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE31280_RAW.tar |
73.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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