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| Status |
Public on Dec 18, 2010 |
| Title |
Search for specific biomarkers of IFN-beta bioactivity in patients with MS |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We aimed to identify specific biomarkers of IFN-beta bioactivity in order to compare their gene expression induction by type I IFNs with the MxA, and to investigate their potential role in MS pathogenesis. Gene expression microarrays were performed in PBMC from MS patients who developed neutralizing antibodies (NAB) to IFN-beta. Nine genes followed patterns in gene expression over time similar to the MX1 and were selected for further experiments: IFI6, IFI27, IFI44L, IFIT1, HERC5, LY6E, RSAD2, SIGLEC1, and USP18. In vitro experiments revealed specific induction of selected biomarkers by IFN-beta but not IFN-gamma, and several markers, in particular USP18 and HERC5, were significantly induced at lower IFN-beta concentrations and more selective than the MX1 as biomarkers of IFN-beta bioactivity. In addition, USP18 expression was deficient in MS patients compared with healthy controls (p=0.0004). We propose specific biomarkers that may be considered in addition to the MxA to evaluate IFN-beta bioactivity, and to further explore their implication in MS pathogenesis.
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| Overall design |
Number of samples: 32. We analyzed PBMC from 8 patients at baseline and after 3, 12 and 24 months of IFN-beta treatment
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| Contributor(s) |
Malhotra S, Bustamante M, Miralles P, Río J, Ruiz de Villa MC, Vegas E, Nonell L, Deisenhammer F, Fissolo N, Montalban X, Comabella M |
| Citation(s) |
21886806 |
| Submission date |
Dec 16, 2010 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Ramil Nurtdinov |
| E-mail(s) |
ramil.nurtdinov@crg.eu
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| Organization name |
Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
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| Department |
Bioinformatics and Genomics
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| Lab |
Computational Biology of RNA Processing
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| Street address |
Dr. Aiguader 88
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| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (32)
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| GSM641345 |
Patient 4, 24 month of BETAFERON |
| GSM641346 |
Patient 13, baseline |
| GSM641347 |
Patient 13, 3 month of REBIF |
| GSM641348 |
Patient 13, 12 month of REBIF |
| GSM641349 |
Patient 13, 24 month of REBIF |
| GSM641350 |
Patient 999, baseline |
| GSM641351 |
Patient 999, 3 month of BETAFERON |
| GSM641352 |
Patient 999, 12 month of BETAFERON |
| GSM641353 |
Patient 999, 24 month of BETAFERON |
| GSM641354 |
Patient 998, baseline |
| GSM641355 |
Patient 998, 3 month of BETAFERON |
| GSM641356 |
Patient 998, 12 month of BETAFERON |
| GSM641357 |
Patient 998, 24 month of BETAFERON |
| GSM641358 |
Patient 108, baseline |
| GSM641359 |
Patient 108, 3 month of BETAFERON |
| GSM641360 |
Patient 108, 12 month of BETAFERON |
| GSM641361 |
Patient 108, 24 month of BETAFERON |
| GSM641362 |
Patient 112, baseline |
| GSM641363 |
Patient 112, 3 month of REBIF |
| GSM641364 |
Patient 112, 12 month of REBIF |
| GSM641365 |
Patient 112, 24 month of REBIF |
| GSM641366 |
Patient 113, baseline |
| GSM641367 |
Patient 113, 3 month of REBIF |
| GSM641368 |
Patient 113, 12 month of REBIF |
| GSM641369 |
Patient 113, 24 month of REBIF |
| GSM641370 |
Patient 116, baseline |
| GSM641371 |
Patient 116, 3 month of REBIF |
| GSM641372 |
Patient 116, 12 month of REBIF |
| GSM641373 |
Patient 116, 24 month of REBIF |
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| Relations |
| BioProject |
PRJNA135181 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26104_RAW.tar |
138.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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