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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 01, 2012 |
| Title |
MDS and DNA repair defects in Crebbp+/- mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Myelodysplastic syndrome (MDS) is considered a disease of hematopoietic stem cell (HSC) origin. To begin to unravel the molecular mechanisms underlying the deregulation of HSCs in MDS, we performed comparative gene expression profiling on Crebbp+/- and wild type HSCs. We chose to isolate HSCs from the fetal liver (FLHSC) because at this stage there were no differences in cell number between Crebbp+/- and wild type fetal livers, suggesting no overt hematopoietic differences. Thus, any change in gene expression found in Crebbp+/- FLHSCs is likely to reflect the initially compromised genetic program of HSC regulation, as opposed to that of Crebbp+/- HSCs in adult bone marrow, where secondary changes in gene expression may also occur as compensatory mechanisms for a compromised or failing hematopoietic system. We used day 14.5 post coitus FLHSC (Sca-1+,Lin-,AA4.1+,c-Kit++) from wild type (wt) and Crebbp heterozygous (ht) embryos to examine changes in gene expression before overt myelodysplastic disease manifestation.
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| Overall design |
Total RNA from wt and Crebbp+/- FLHSCs was isolated, PCR-amplified using the Ovation RNA amplification system and hybridized to Affymetrix Mouse 430 2.0 expression microarrays.
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| Contributor(s) |
Lemieux ME |
| Citation(s) |
22198154 |
| Submission date |
Sep 10, 2009 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Madeleine E. Lemieux |
| E-mail(s) |
mlemieux@bioinfo.ca
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| Phone |
617-595-6695
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| URL |
http://www.bioinfo.ca
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| Organization name |
Bioinfo
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| Street address |
275 Main St., Suite 252
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| City |
Plantagenet |
| State/province |
ON |
| ZIP/Postal code |
K0B 1L0 |
| Country |
Canada |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA119327 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE18061_RAW.tar |
26.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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