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Series GSE1548

Query DataSets for GSE1548

Status

Public on Aug 31, 2004

Title

Effect of Angiotensin II on gene expression in SHRSP cerebral microcapillaries

Organism

Rattus norvegicus

Experiment type

Expression profiling by array

Summary

Renin-angiotensin system (RAS) inhibition reduces stroke and improves brain capillary integrity in stroke prone spontaneously hypertensive rats (SHRSP). We tested the hypothesis that treatment with an angiotensin II receptor subtype 1 (AT1R) antagonist has different effects, compared to an angiotensin converting enzyme (ACE) inhibitor, on gene expression in blood-brain barrier (BBB) capillaries. Six weeks old SHRSP were treated with either olmesartan (4 mg/kg, n=20), lisinopril (6 mg/kg , n=20) or remained untreated (n=20). Blood pressure was controlled by tail-cuff measurement. After 5 weeks the animals were sacrificed and cerebral capillaries were isolated. mRNA was extracted and analyzed with rat GeneChip DNA arrays. Additionally, brain histology and monocyte/macrophage infiltrates were determined. Both treatments similarly reduced neurological signs of stroke, stroke mortality, and monocyte/macrophage infiltration, compared to controls. Blood pressure was not influenced significantly by both drugs. We found 42 transcripts that were regulated by both treatments in the same manner. These genes were mostly related to inflammation. We also observed 39 differentially expressed genes between the two treatment groups that typically contribute to cell growth and differentiation. This study demonstrates that, despite similar effects on cerebral pathology and outcome, ACE inhibition and AT1R blockade have distinct molecular effects on gene expression in BBB capillaries.
Keywords = angiotensin II
Keywords = gene expression
Keywords = microarrays
Keywords = brain capillaries
Keywords = SHRSP
Keywords = olmesartan
Keywords = lisinopril
Keywords: other

Contributor(s)

Kirsch T, Wellner M, Gueler F, Park J, Beese M, Haller H, Lippoldt A

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Submission date

Jul 08, 2004

Last update date

Feb 21, 2017

Contact name

Torsten Kirsch

E-mail(s)

TKirsch@gmx.net