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| Status |
Public on Mar 25, 2008 |
| Title |
Histone deacetylase 4 represses dystrophin-glycoprotein (DGC) complex expression |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Dysfunction of the dystrophin-glycoprotein complex (DGC) is a frequent cause of hereditary forms of muscular dystrophy. Although DGC function in maintaining skeletal muscle integrity has been well characterized, little is known about how the DGC complex is coordinately regulated at the transcriptional level. To test this hypothesis, we engineered HDAC4 stably overexpressing and control myotubes in an in vitro model of muscle differentiation. Here we present evidence that HDAC4, a neural activity-responsive histone deacetylase, is a critical transcriptional regulator of the DGC complex. We show that HDAC4 can repress multiple components of the DGC complex, including dystrophin and sarcoglycan family members in both cultured myotubes. To confirm this finding, the protein levels of core DGC complex members including dystrophin, sarcoglycan complex members, and additional dystrophin-associated proteins were evaluated in differentiated myotubes by western analysis.
Keywords: genetic modification and cell type comparison of muscle cells
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| Overall design |
C2C12 mouse myotubes were infected with either a HDAC4 expressing or control (Neo) retroviruses. After stable selection, myotubes were differentiated at 90% confluency in 2% horse serum (Hyclone) for 4 days. At this time point, RNA was extracted and the two types of cells compared in a microarray analysis.
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| Contributor(s) |
Cohen TJ, Barrientos T, Garvey SM, Hartman ZC, Cox GA, Bedlack RS, Yao T |
| Citation(s) |
18780762 |
| Submission date |
Feb 29, 2008 |
| Last update date |
Mar 18, 2013 |
| Contact name |
Zachary Conrad Hartman |
| E-mail(s) |
zch@duke.edu
|
| Phone |
919-684-9197
|
| Organization name |
Duke University
|
| Department |
Surgery
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| Lab |
Lyerly Lab
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| Street address |
Research Drive MSRB rm 414
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| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
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| Platforms (1) |
| GPL3222 |
Duke University Murine Operon v.3.0 spotted Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA107661 |
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