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Status
Public on Feb 17, 2006
Title
Buck Institute Human 8k cDNA microarray
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Homo sapiens Manufacturer
Genomics Facility, Buck Institute for Age Research
Manufacture protocol
8,432 randomly chosen cDNAs from a 40k Research Genetics human cDNA library were amplified under standard conditions (GeneAmp PCR System 9700) with the help of a volumetric robot (Tecan/Genesis, RSP150 and Tomtec/Quadra 384). Amplified products were transferred to a 384-well plates, purified transferred to 384-well print plates (Genetix), dried and resuspended in print buffer (3xSSC, 1.5M Betaine). Spotted cDNA microarrays were generated on glass slides (MWC epoxy) using an Omnigene (GeneMachines) microarrayer. After printing, slides were incubated overnight at 50% humidity, UV irradiated (65 ujoules), and stored in a desicator until hybridization.
Contributor(s)
Mahoney DJ , Parise G , Melov S , Safdar A , Tarnopolsky MA Citation(s)
15985525 Submission date
Feb 15, 2006
Last update date
Feb 17, 2006
Contact name
Douglas Joseph Mahoney
E-mail(s)
doug@mgcheo.med.uottawa.ca Phone
613 738 3927
Fax
613 738 4833
Organization name
CHEO Research Institute
Department
Apoptosis Research Center
Street address
401 Smyth Rd
City
Ottawa
State/province
ON
ZIP/Postal code
K1H 8L1
Country
Canada
Samples (15)
GSM96804 , GSM96806 , GSM96807 , GSM96808 , GSM96809 , GSM96811
Series (3)
GSE4247 cDNA microarray analysis of human skeletal muscle after endurance exercise
GSE4249 cDNA microarray analysis of human skeletal muscle after eccentric exercise
GSE4252 cDNA microarray analysis of human skeletal muscle after eccentric or endurance exercise
Data table header descriptions ID GB_ACC GeneBank accession number
Unigene cluster ID Unigene_Hs cluster identifier
Description Unigene title
Data table ID GB_ACC Unigene cluster ID Description 1
AA001222 561654
Olfactory receptor, family 2, subfamily A, member 9 pseudogene
2
AA001359 571599
MRNA; cDNA DKFZp686B1082 (from clone DKFZp686B1082)
3
AA001360 4
AA001403 132642
Zinc metallopeptidase (STE24 homolog, yeast)
5
AA001444 526754
Meis1, myeloid ecotropic viral integration site 1 homolog (mouse)
6
AA001536 69749
KIAA0087 gene product
7
AA001604 423725
Zinc finger with KRAB and SCAN domains 1
8
AA001611 254642
Hypothetical protein FLJ11506
9
AA001614 10
AA001639 189920
Polybromo 1
11
AA001658 458462
Dehydrogenase/reductase (SDR family) X-linked
12
AA001718 13
AA001749 472437
Microtubule-associated protein, RP/EB family, member 1
14
AA001835 269211
Zinc finger protein 262
15
AA001839 16
AA001841 17
AA001870 520246
KIAA1117
18
AA001876 19
AA001897 119825
Spectrin, alpha, erythrocytic 1 (elliptocytosis 2)
20
AA001918 21757
Mesoderm induction early response 1 homolog (Xenopus laevis)
8008 429 Kbytes .Supplementary data files not provided
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