GEO DataSets

Analysis of liver from C57BL/6 adult males treated with an antisense oligonucleotide (ASO) targeting liver-specific microRNA (miRNA) miR-122. miR-122 is expressed at high levels in the adult liver, making up 70% of all miRNA. Results provide insight into the role of miR-122 in the adult liver.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL2897

Series:

GSE3603

10 Samples

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(Submitter supplied) Current understanding of microRNA (miRNA) biology is limited, and antisense oligonucleotide (ASO) inhibition of miRs is a powerful technique for their functionalization. To uncover the role of the liver-specific miR-122 in the adult liver, we inhibited it in vivo using a 2’-O-methoxyethyl phosphorothioate ASO. This microrarray experiment was used to identify changes in mRNA levels due to the inhibition of miR-122 that accompanied phenotypic observations of reduced cholesterol and triglycerides, increased fatty acid oxidation, and decreased fatty acid and cholesterol synthesis rates observed in hepatocytes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1729

Platform:

GPL2897

10 Samples

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(Submitter supplied) Using a novel class of chemically-engineered oligonucleotides, termed "antagomirs", we studied the biological significance of silencing miR-122 in the liver of mice at the mRNA level Keywords: treatment response

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

2 Samples

Download data: CEL

(Submitter supplied) Antagonism of microRNA-122 in mice by systemically administered LNA-antimiR leads to up-regulation of a large set of predicted target mRNAs in the liver MicroRNA-122 (miR-122) is an abundant liver-specific miRNA, implicated in fatty acid and cholesterol metabolism as well as hepatitis C viral replication. Here, we report that a systemically administered 16-nt, unconjugated LNA (locked nucleic acid)-antimiR oligonucleotide complementary to the 5' end of miR-122 leads to specific, dose-dependent silencing of miR-122 and shows no hepatotoxicity in mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3805

Platform:

GPL1261

21 Samples

Download data: CEL

Analysis of liver from NMRI females treated with 25mg/kg LNA (locked nucleic acid)-antimiR-122 on day 2 and sacrificed at days 3, 9 and 23. Results provide insight into the utility of LNA-modified oligonucleotides in silencing miRNAs in vivo.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 agent, 3 time sets

Platform:

GPL1261

Series:

GSE13948

21 Samples

Download data: CEL

(Submitter supplied) Acute lymphoblastic leukemia (ALL) is an heterogeneous disease comprising several subentities that differ for both immunophenotypic and molecular characteristics. Over the years, the biologic understanding of this neoplasm has largely increased. Gene expression profiling has recently allowed to identify specific signatures for the different ALL subsets and permitted identification of pathways deregulated by a given lesion. more...

Organism:

Homo sapiens

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL570 GPL8191

39 Samples

Download data: CEL, XLS

(Submitter supplied) Transcriptional profiling of mouse livers comparing control mice with miR-155 transgenic mice that overexpression of miR-155 in the liver

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13509

6 Samples

Download data: LSR, XLS

(Submitter supplied) The effects of high-fat (HF) feeding on the gene expression in the small intestine were examined using obesity-resistant A/J mice and obesity-sensitive C57BL/6J (B6) mice. Both strains of mice were maintained on low-fat (LF, 5% fat) or HF (30% fat) diets for two weeks. Oligonucleotide microarray and semi-quantitative RT-PCR analysis revealed that lipid metabolism-related genes including mitochondrial acyl-CoA thioesterase 1, pyruvate dehydrogenase kinase 4, and NADP+-dependent cytosolic malic enzyme were up-regulated by more than 2-fold in both strains of mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2909

Platform:

GPL339

4 Samples

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Analysis of small intestines of A/J and C57BL/6J animals on a low or high fat diet. The A/J strain is resistant to obesity, C57BL/6J is prone. Results provide insight into the factors contributing to the susceptibility to obesity.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 protocol, 2 strain sets

Platform:

GPL339

Series:

GSE3433

4 Samples

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(Submitter supplied) To evaluate wether miRNA expression patterns contributes to obesity total RNA were purified from subcutaneous adipose tissue (SAT)and used in miRNA microarrays. Platform contain LNA-modified probes for all human miRNAs present in release 8.2 of the miRBase microRNA Registry. Two RNA pools from 3 non obese men (CM) and from 5 non obese women (CW) were used as controls. Expression profiling revealed that a large set of miRNAs is expressed in SAT. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL7162

20 Samples

Download data

(Submitter supplied) Exiqon design 208002V8.1 Lot: 20393.03 http://www.exiqon.com Protocol: See manufacturer's web site at http://www.exiqon.com

Organism:

Homo sapiens

1 Series

20 Samples

Download data: GAL

(Submitter supplied) Transcriptional profiling of mouse liver comparing control, scrambled-oligonucleotides (Cont)-treated mice with Perilipin 2-antisense oligonucleotides (Plin2-ASO)-treated mice. C57BL/6 mice on a high-fat diet were treated with oligonucleotides in vivo. The goal was to determine the effects of Plin2 down-regulation in the liver on global gene expression.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

4 Samples

Download data: TXT

(Submitter supplied) MicroRNAs (miRNAs) are important regulators and potential therapeutic targets of metabolic disease. In this study we show by in vivo administration of locked nucleic acid (LNA) inhibitors that suppression of endogenous miR-29 lowers plasma cholesterol levels by ~40%, commensurate with the effect of statins, and reduces fatty acid content in the liver by ~20%. Whole transcriptome sequencing of the liver reveals 883 genes dysregulated (612 down, 271 up) by inhibition of miR-29. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

12 Samples

Download data: TXT