Isolation of two human cDNAs, HLP3 and HLP4, homologous to the neuron-specific calcium-binding protein genes

M Kobayashi; E Sakai; Y Furuta; K Takamatsu

doi:10.3109/10425179809072192

Isolation of two human cDNAs, HLP3 and HLP4, homologous to the neuron-specific calcium-binding protein genes

DNA Seq. 1998;9(3):171-6. doi: 10.3109/10425179809072192.

Authors

M Kobayashi¹, E Sakai, Y Furuta, K Takamatsu

Affiliation

¹ Department of Physiology, Toho University School of Medicine, Tokyo, Japan.

PMID: 10520747
DOI: 10.3109/10425179809072192

Abstract

We have cloned and sequenced two types of human cDNA, HLP3 and HLP4, encoding a protein of 191 amino acid residues with four EF-hand calcium-binding motifs. The HLP3 and HLP4 proteins are homologous to the neuron-specific calcium-binding proteins and are likely human counterparts of the neural visinin-like protein(NVP) 1 and NVP2 identified in the rat brain (Kajimoto et al. 1993), displaying 98% and 99% amino acid identities with these sequences, respectively. The human HLP3 and 4 mRNAs are detected only in the brain and found at high amount in the cerebral cortex and cerebellum by Northern blot analysis.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Brain / metabolism
Calcium-Binding Proteins / genetics*
Cloning, Molecular
DNA, Complementary / genetics*
DNA, Complementary / isolation & purification
EF Hand Motifs / genetics
Gene Expression
Humans
Molecular Sequence Data
Nerve Tissue Proteins / genetics*
Neurocalcin
RNA, Messenger / genetics
RNA, Messenger / metabolism
Rats
Receptors, Calcium-Sensing*
Tissue Distribution

Substances

Calcium-Binding Proteins
DNA, Complementary
NVP2 protein, rat
Nerve Tissue Proteins
Neurocalcin
RNA, Messenger
Receptors, Calcium-Sensing
VSNL1 protein, human
Vsnl1 protein, rat

Associated data

GENBANK/AB001104
GENBANK/AB001105