Structure and expression of human fibroblast growth factor-10

J Biol Chem. 1997 Sep 12;272(37):23191-4. doi: 10.1074/jbc.272.37.23191.

Abstract

We isolated the cDNA encoding a novel member of the human fibroblast growth factor (FGF) family from the lung. The cDNA encodes a protein of 208 amino acids with high sequence homology (95.6%) to rat FGF-10, indicating that the protein is human FGF-10. Human FGF-10 as well as rat FGF-10 has a hydrophobic amino terminus ( approximately 40 amino acids), which may serve as a signal sequence. The apparent evolutionary relationships of human FGFs indicate that FGF-10 is closest to FGF-7. Chromosomal localization of the human FGF-10 gene was examined by in situ hybridization. The gene was found to map to the 5p12-p13 region. Human FGF-10 (amino acids 40 to 208 with a methionine residue at the amino terminus) was produced in Escherichia coli and purified from the cell lysate. Recombinant human FGF-10 (approximately 19 kDa) showed mitogenic activity for fetal rat keratinizing epidermal cells, but essentially no activity for NIH/3T3 cells, fibroblasts. The specificity of mitogenic activity of FGF-10 is similar to that of FGF-7 but distinct from that of bFGF. In structure and biological activity, FGF-10 is similar to FGF-7.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 5 / genetics
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Evolution, Molecular
  • Fibroblast Growth Factor 10
  • Fibroblast Growth Factors / biosynthesis
  • Fibroblast Growth Factors / genetics*
  • Fibroblast Growth Factors / pharmacology
  • Humans
  • In Situ Hybridization
  • Lung / chemistry
  • Mitogens / genetics
  • Molecular Sequence Data
  • Multigene Family
  • Recombinant Proteins / biosynthesis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Species Specificity

Substances

  • FGF10 protein, human
  • Fibroblast Growth Factor 10
  • Mitogens
  • Recombinant Proteins
  • Fibroblast Growth Factors

Associated data

  • GENBANK/AB002097