LAIR-1, a novel inhibitory receptor expressed on human mononuclear leukocytes

L Meyaard; G J Adema; C Chang; E Woollatt; G R Sutherland; L L Lanier; J H Phillips

doi:10.1016/s1074-7613(00)80530-0

LAIR-1, a novel inhibitory receptor expressed on human mononuclear leukocytes

Immunity. 1997 Aug;7(2):283-90. doi: 10.1016/s1074-7613(00)80530-0.

Authors

L Meyaard¹, G J Adema, C Chang, E Woollatt, G R Sutherland, L L Lanier, J H Phillips

Affiliation

¹ Department of Immunobiology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304-1104, USA.

PMID: 9285412
DOI: 10.1016/s1074-7613(00)80530-0

Abstract

In the present study, we describe a novel inhibitory receptor, leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1), that is constitutively expressed on the majority of human peripheral blood mononuclear leukocytes. LAIR-1 is a 32 kDa transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs. LAIR-1 recruits SHP-1 and SHP-2 phosphatases upon activation, and cross-linking of the LAIR-1 antigen on natural killer (NK) cells results in strong inhibition of NK cell-mediated cytotoxicity. Although it is structurally related to human killer cell inhibitory receptors, LAIR-1 does not appear to recognize human leukocyte antigen (HLA) class I molecules and thus represents a novel HLA class I-independent mechanism of NK cell regulation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA, Complementary / isolation & purification
HL-60 Cells
Humans
Intracellular Signaling Peptides and Proteins
Jurkat Cells
Killer Cells, Natural / metabolism
Leukocytes, Mononuclear / metabolism*
Molecular Sequence Data
Protein Binding / immunology
Protein Tyrosine Phosphatase, Non-Receptor Type 11
Protein Tyrosine Phosphatase, Non-Receptor Type 6
Protein Tyrosine Phosphatases / metabolism
RNA, Messenger / biosynthesis
Receptors, Immunologic / biosynthesis*
Receptors, Immunologic / chemistry
Receptors, Immunologic / genetics
Receptors, Immunologic / physiology*
Tumor Cells, Cultured

Substances

DNA, Complementary
Intracellular Signaling Peptides and Proteins
RNA, Messenger
Receptors, Immunologic
leukocyte-associated immunoglobulin-like receptor 1
PTPN11 protein, human
PTPN6 protein, human
Protein Tyrosine Phosphatase, Non-Receptor Type 11
Protein Tyrosine Phosphatase, Non-Receptor Type 6
Protein Tyrosine Phosphatases

Associated data

GENBANK/AF013249
GENBANK/AF013250