Abstract
The murine cell-surface antigen MS2 [ADAM 8; mouse CD156 (mCD156)] is a transmembrane glycoprotein that is highly expressed in monocytic lineages. mCD156 consists of a long exterior region containing domains strikingly similar to those of hemorrhagic snake venom proteins. cDNA for human CD156 was isolated from cDNA libraries from the human macrophage cell line THP-1 and from human granulocytes. The CD156 cDNA detected mRNA from human macrophage cell lines, granulocytes, monocytes, and B cell but not T cell lines. The nucleotide sequence of the CD156 cDNA showed 65.6% homology with that of mCD156, and its amino acid sequence had high homology with hemorrhagic snake venom proteins and other related mammalian proteins. The CD156 gene was mapped to human chromosome 10q26.3.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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ADAM Proteins
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Amino Acid Sequence
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Animals
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Antigens, CD*
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Antigens, Surface / genetics*
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Cell Line
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Chromosome Mapping
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Chromosomes, Human, Pair 10 / genetics
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Cloning, Molecular
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Consensus Sequence
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Gene Expression
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Humans
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In Situ Hybridization, Fluorescence
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Leukocytes / immunology
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Membrane Proteins / genetics*
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Metalloendopeptidases*
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Mice
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Molecular Sequence Data
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Sequence Homology, Amino Acid
Substances
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Antigens, CD
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Antigens, Surface
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Membrane Proteins
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RNA, Messenger
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ADAM Proteins
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ADAM8 protein, human
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Adam8 protein, mouse
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Metalloendopeptidases
Associated data
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GENBANK/D14665
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GENBANK/D26579
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GENBANK/D31872
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GENBANK/D50410
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GENBANK/X13335
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GENBANK/X64227
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GENBANK/X66140
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GENBANK/X68251
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GENBANK/Z11719
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GENBANK/Z11720