Abstract
cDNA encoding a novel human ubiquitin-conjugating enzyme has been cloned from an epidermoid carcinoma KB cDNA library. This clone encodes a protein of 152 amino acids with a calculated M(r) of 17,137. The amino acid sequence showed 80% identity with the Drosophila's bendless gene product (ubiquitin-conjugating enzyme E2). The corresponding transcripts are highly expressed in heart, skeletal muscle, and testis. The product expressed in Escherichia coli exhibited the ability to form a thiol ester linkage with ubiquitin in a ubiquitin-activating enzyme E1-dependent manner. These results suggest that the obtained cDNA encodes a novel human E2 which may be involved in protein degradation mainly in the muscles and testis.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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DNA, Complementary
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Drosophila / enzymology*
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Drosophila / genetics
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Drosophila Proteins*
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Escherichia coli
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Female
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Gene Expression*
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Humans
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KB Cells
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Ligases / biosynthesis*
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Ligases / chemistry*
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Male
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Molecular Sequence Data
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Muscle, Skeletal / enzymology
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Myocardium / enzymology
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Organ Specificity
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Restriction Mapping
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Testis / enzymology
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Transcription, Genetic
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Ubiquitin-Conjugating Enzymes
Substances
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DNA, Complementary
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Drosophila Proteins
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Ubiquitin-Conjugating Enzymes
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ben protein, Drosophila
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Ligases
Associated data
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GENBANK/D83004
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GENBANK/H10694
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GENBANK/H84799
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GENBANK/H98983
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GENBANK/N39781
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GENBANK/N44818
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GENBANK/N49129
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GENBANK/N84246
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GENBANK/R54347
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GENBANK/R83392
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GENBANK/T23480
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GENBANK/T30096
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GENBANK/T30100
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GENBANK/T32724
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GENBANK/T83898
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GENBANK/T96902
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GENBANK/T98324
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GENBANK/T99403
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GENBANK/T99509
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GENBANK/W00674
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GENBANK/W03614
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GENBANK/W05757
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GENBANK/Z50111