Molecular cloning and RNA expression of two new human chemokine receptor-like genes

Biochem Biophys Res Commun. 1996 Oct 23;227(3):846-53. doi: 10.1006/bbrc.1996.1595.

Abstract

Two new human genes encoding putative G protein-coupled receptors were cloned from genomic DNA following a degenerate PCR strategy. The predicted amino acid sequences of the proteins encoded by these genes have the characteristic motifs of chemokine receptors. This prompted us to name these genes CKR-L1 and CKR-L3. Nevertheless, the identification of their ligands has not been possible using 16 human chemokines in three different assays. The RNA expression pattern of CKR-L1 and CKR-L3 is also similar to that generally found for chemokine receptors. To a different extent, these genes are expressed in spleen, lymph nodes, and CD4+, CD8+ and CD19+ lymphocytes. CKR-L1 expression was also detected in monocyte/macrophages. The homology to chemokine receptors suggests that CKR-L1 belongs to the family of beta chemokine receptors, while CKR-L3 is more similar to alpha chemokine receptors.

MeSH terms

  • Amino Acid Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • Humans
  • Ligands
  • Molecular Sequence Data
  • RNA / genetics*
  • Receptors, CCR6
  • Receptors, CCR8
  • Receptors, Chemokine*
  • Receptors, Cytokine / genetics*
  • Receptors, Cytokine / metabolism
  • Sequence Homology, Amino Acid

Substances

  • CCR6 protein, human
  • CCR8 protein, human
  • Ligands
  • Receptors, CCR6
  • Receptors, CCR8
  • Receptors, Chemokine
  • Receptors, Cytokine
  • RNA

Associated data

  • GENBANK/Z79782
  • GENBANK/Z79784