Human cardiotrophin-1: protein and gene structure, biological and binding activities, and chromosomal localization

Cytokine. 1996 Mar;8(3):183-9. doi: 10.1006/cyto.1996.0026.

Abstract

Cardiotrophin-1 (CT-1) is a new member of the interleukin-6 cytokine family that was identified from a mouse embryoid body cDNA library by expression cloning. Mouse CT-1 induces features of hypertrophy in neonatal rat cardiac myocytes and binds to and activates the leukaemia inhibitory factor/gp130 receptor complex. In this work we report the isolation and characterization of cDNA and genomic clones encoding human CT-1. These clones encode a 201 amino acid protein that is 80% identical to the mouse protein. Human CT-1 produced by transfection of the cDNA clones into mammalian cells induces the hypertrophy of neonatal rat cardiac myocytes. Human and mouse CT-1 bind to the leukaemia inhibitory factor receptor on both human and mouse cell lines indicating a lack of species specificity. No binding to the human oncostatin M specific receptor was detected. A 1.7 kb CT-1 mRNA is expressed in adult human heart, skeletal muscle, ovary, colon, prostate and testis and in fetal kidney and lung. The coding region of CT-1 is contained on three exons and is located on human chromosome 16p11.1-16p11.2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Chromosome Mapping
  • Chromosomes, Human, Pair 16*
  • Cloning, Molecular
  • Consensus Sequence
  • Cytokines / chemistry*
  • Cytokines / genetics*
  • Cytokines / metabolism
  • DNA, Complementary
  • Exons
  • Female
  • Gene Library
  • HeLa Cells
  • Humans
  • Hybrid Cells
  • In Situ Hybridization, Fluorescence
  • Male
  • Mice
  • Molecular Sequence Data
  • Organ Specificity
  • RNA, Messenger / biosynthesis
  • Rats
  • Receptors, Cytokine / metabolism
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Transfection

Substances

  • Cytokines
  • DNA, Complementary
  • RNA, Messenger
  • Receptors, Cytokine
  • Recombinant Proteins
  • cardiotrophin 1

Associated data

  • GENBANK/U43030