Abstract
We have isolated a novel human cDNA with a predicted amino acid sequence homologous to GCN5, a protein considered to be a regulator of transcriptional activation in yeast. This cDNA, termed GCN5L1 (GCN5-like 1), consists of 545 nucleotides including an open reading frame of 378 bp that encodes a 126-amino-acid peptide having 23.5% identity to yeast GCN5. Northern-blot analysis revealed transcription of this gene in all human tissues examined. We isolated a genomic clone corresponding to this cDNA from a human cosmid library and mapped it to chromosome 12q13 --> q14 by fluorescent in situ hybridization (FISH).
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Chromosomes, Human, Pair 12 / genetics*
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Cloning, Molecular
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Cosmids
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DNA, Complementary / genetics*
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DNA-Binding Proteins*
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Fungal Proteins / genetics*
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Gene Expression
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Gene Library
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Genes*
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Genes, Fungal
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Histone Acetyltransferases
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Humans
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In Situ Hybridization, Fluorescence
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Molecular Sequence Data
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Nerve Tissue Proteins*
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Open Reading Frames
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Protein Kinases / genetics*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Species Specificity
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Trans-Activators / genetics*
Substances
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BLOC1S1 protein, human
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DNA, Complementary
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DNA-Binding Proteins
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Fungal Proteins
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Nerve Tissue Proteins
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Saccharomyces cerevisiae Proteins
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Trans-Activators
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GCN5 protein, S cerevisiae
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Histone Acetyltransferases
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Protein Kinases