Abstract
Based on protein sequence, we have isolated a cDNA for intercellular adhesion molecule 3 (ICAM-3), the most recently defined counter-receptor for lymphocyte function-associated antigen 1 (LFA-1). Expression of the cDNA yields a product that reacts with monoclonal antibody to ICAM-3 and functions as a ligand for LFA-1. The deduced 518-amino acid sequence of the predicted mature protein defines a highly glycosylated type I integral membrane protein with five immunoglobulin (Ig)-like domains. The five Ig-like domains of ICAM-3 are highly homologous with those of human ICAM-1 (52% identity) and human ICAM-2 (37% identity).
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, CD*
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Antigens, Differentiation*
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Base Sequence
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Cell Adhesion Molecules / chemistry
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Cell Adhesion Molecules / genetics*
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Cell Adhesion Molecules / metabolism
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Cloning, Molecular
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DNA
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Electrophoresis, Polyacrylamide Gel
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Flow Cytometry
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Humans
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Immunoglobulins / chemistry*
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Lymphocyte Function-Associated Antigen-1 / metabolism*
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Mice
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Molecular Sequence Data
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Receptors, Antigen / chemistry
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Receptors, Antigen / genetics*
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Receptors, Antigen / metabolism
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Restriction Mapping
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Sequence Homology, Amino Acid
Substances
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Antigens, CD
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Antigens, Differentiation
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Cell Adhesion Molecules
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ICAM3 protein, human
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Immunoglobulins
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Lymphocyte Function-Associated Antigen-1
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Receptors, Antigen
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DNA