Abstract
The gene for human aminomethyltransferase (AMT), also known as the T-protein of the glycine cleavage system, was isolated from a human placental cosmid library and examined by restriction mapping, polymerase chain reaction analysis, and DNA sequencing. The gene is about 6 kb in length and consists of nine exons. The 5'-flanking region of the gene lacks typical TATAA sequence but has a single defined transcription initiation site detected by the primer extension method. Two putative glucocorticoid-responsive elements and a putative thyroid hormone-responsive element are present. The AMT gene was assigned to subband 3p21.2-p21.1 by fluorescence in situ hybridization.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Metabolism, Inborn Errors / genetics*
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Aminomethyltransferase
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Base Sequence
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Chromosome Mapping
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Chromosomes, Human, Pair 3*
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Exons
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Genes*
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Glycine / blood*
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Humans
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Hydroxymethyl and Formyl Transferases*
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In Situ Hybridization, Fluorescence
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Molecular Sequence Data
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Multienzyme Complexes
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Polymerase Chain Reaction
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Regulatory Sequences, Nucleic Acid
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Transferases / deficiency
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Transferases / genetics*
Substances
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Multienzyme Complexes
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Transferases
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Hydroxymethyl and Formyl Transferases
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Aminomethyltransferase
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Glycine
Associated data
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GENBANK/D14681
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GENBANK/D14682
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GENBANK/D14684
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GENBANK/D14685
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GENBANK/D14686