Physical and genetic localization of a Shab subfamily potassium channel (KCNB1) gene to chromosomal region 20q13.2

Genomics. 1995 Jan 1;25(1):285-7. doi: 10.1016/0888-7543(95)80138-c.

Abstract

A human delayed rectifier K+ channel gene has been localized to the long arm of human chromosome 20q13.2 by fluorescence in situ hybridization of genomic P1 clones from this locus. A polymorphic (GA) microsatellite repeat was identified in one of the P1 clones. The new SSR marker (D20S436) was genotyped in four CEPH pedigrees. Two-point linkage analysis indicated linkage of this marker to a PCR marker, D20S109, with a maximum lod score of 9.32 at theta = 0.001. The assignment of this K+ channel gene to 20q13.2 eliminates it as a candidate for the gene associated with benign familial neonatal convulsions (BFNC), which has been localized to 20q13.3. Genetically, the K+ channel gene maps more than 30 cM proximal to the BFNC locus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 20*
  • Cloning, Molecular
  • DNA Primers
  • Genetic Markers
  • Genomic Library
  • Humans
  • In Situ Hybridization, Fluorescence
  • Lymphocytes / cytology
  • Lymphocytes / metabolism
  • Male
  • Molecular Sequence Data
  • Multigene Family*
  • Polymerase Chain Reaction
  • Potassium Channels / genetics*
  • Shab Potassium Channels

Substances

  • DNA Primers
  • Genetic Markers
  • KCNB1 protein, human
  • Potassium Channels
  • Shab Potassium Channels

Associated data

  • GENBANK/X68302