Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3), and 40-kDa (RFC2) subunit genes of human replication factor C to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23

K Okumura; M Nogami; H Taguchi; F B Dean; M Chen; Z Q Pan; J Hurwitz; A Shiratori; Y Murakami; K Ozawa

doi:10.1016/0888-7543(95)80135-9

Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3), and 40-kDa (RFC2) subunit genes of human replication factor C to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23

Genomics. 1995 Jan 1;25(1):274-8. doi: 10.1016/0888-7543(95)80135-9.

Authors

K Okumura¹, M Nogami, H Taguchi, F B Dean, M Chen, Z Q Pan, J Hurwitz, A Shiratori, Y Murakami, K Ozawa, et al.

Affiliation

¹ Division of Human Genome Research and Gene Bank, Tsukuba Life Science Center, Ibaraki, Japan.

PMID: 7774928
DOI: 10.1016/0888-7543(95)80135-9

Abstract

Replication factor C is a multimeric primer-recognition protein consisting of five subunits (p145, p40, p38, p37, and p36.5) and is essential for the processive elongation of DNA chains catalyzed by DNA polymerase delta or epsilon in human cells. We have mapped the locations on human chromosomes of the genes coding for the four smaller subunits [p36.5 (RFC5), p37 (RFC4), p38 (RFC3), and p40 (RFC2)] using both PCR amplification from DNAs of a panel of somatic hybrids and fluorescence in situ hybridization to bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23, respectively.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Chromosome Banding
Chromosome Mapping
Chromosomes, Human, Pair 12*
Chromosomes, Human, Pair 13*
Chromosomes, Human, Pair 3*
Chromosomes, Human, Pair 7*
Cloning, Molecular
DNA Polymerase II
DNA Polymerase III
DNA Primers
DNA Replication / genetics*
DNA-Binding Proteins / biosynthesis
DNA-Binding Proteins / genetics*
DNA-Directed DNA Polymerase / metabolism
Genomic Library
Homeodomain Proteins*
Humans
In Situ Hybridization, Fluorescence
Lymphocytes / cytology
Macromolecular Substances
Minor Histocompatibility Antigens
Molecular Sequence Data
Polymerase Chain Reaction
Proto-Oncogene Proteins c-bcl-2*
Replication Protein C
Repressor Proteins*
Saccharomyces cerevisiae Proteins*
Sequence Tagged Sites

Substances

BCL2-related protein A1
DNA Primers
DNA-Binding Proteins
Homeodomain Proteins
MATA1 protein, S cerevisiae
Macromolecular Substances
Minor Histocompatibility Antigens
Proto-Oncogene Proteins c-bcl-2
RFC2 protein, human
RFC3 protein, human
RFC4 protein, human
RFC5 protein, human
Repressor Proteins
Saccharomyces cerevisiae Proteins
DNA Polymerase II
DNA Polymerase III
DNA-Directed DNA Polymerase
Replication Protein C