Abstract
A cDNA clone encoding bone morphogenetic protein-4 (BMP-4) has been isolated from a human placental cDNA library. Sequence analysis of this clone revealed that the nucleotide sequence of 5' region was different from that of human osteosarcoma BMP-4 and the deduced amino acid sequence indicated deletion of N-terminal 6 amino acids. We confirmed the expression of this type of BMP-4 mRNA in one human osteogenic cell line in addition to the placenta by the polymerase chain reaction (PCR).
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Bone Morphogenetic Proteins
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Bone Neoplasms / metabolism
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Cell Line
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Cloning, Molecular
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DNA Primers
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DNA, Complementary
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Electrophoresis, Polyacrylamide Gel
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Gene Library*
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Growth Substances / biosynthesis
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Growth Substances / genetics*
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Humans
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Mice
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Molecular Sequence Data
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Osteosarcoma / genetics
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Osteosarcoma / metabolism
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Placenta / metabolism*
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Polymerase Chain Reaction
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Protein Biosynthesis
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Proteins / genetics*
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RNA, Messenger / analysis
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Sequence Alignment
Substances
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Bone Morphogenetic Proteins
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DNA Primers
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DNA, Complementary
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Growth Substances
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Proteins
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RNA, Messenger