Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity

P W Gray; B B Aggarwal; C V Benton; T S Bringman; W J Henzel; J A Jarrett; D W Leung; B Moffat; P Ng; L P Svedersky

doi:10.1038/312721a0

Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity

Nature. 1984;312(5996):721-4. doi: 10.1038/312721a0.

Authors

P W Gray, B B Aggarwal, C V Benton, T S Bringman, W J Henzel, J A Jarrett, D W Leung, B Moffat, P Ng, L P Svedersky, et al.

PMID: 6334807
DOI: 10.1038/312721a0

Abstract

A chemically-synthesized gene and natural complementary DNA coding for human lymphotoxin were isolated and engineered for expression in Escherichia coli. Purified recombinant lymphotoxin shows cytotoxic activity on murine and human tumour cell lines in vitro and causes necrosis of certain murine sarcomas in vivo.

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cells, Cultured
Cloning, Molecular
DNA / genetics
Gene Expression Regulation
Humans
Lymphokines / genetics
Lymphotoxin-alpha / genetics*
Lymphotoxin-alpha / therapeutic use
Mice
Sarcoma, Experimental / therapy

Substances

Lymphokines
Lymphotoxin-alpha
DNA

Associated data

GENBANK/X01393