Background: Metastasis is the main cause of death in patients with lung cancer. Macrophages are innate immune cells that play important roles in cancer metastasis. Exosomes could play an important role of communication between tumor cells and macrophages. This study investigated the effect of miR-10b on cell growth invasion and epithelial mesenchymal transition (EMT) in lung adenocarcinoma A549 cell exosomes.
Methods: Exosomes were isolated from A549 cells and identified by transmission electron microscopy (TEM) and Western blot. CCK-8 assay and flow cytometry were used to detect cell proliferation and apoptosis. Cell migration and invasion were detected by Transwell assay. The expression of mRNA and protein were assessed by quantitative reverse transcription polymerase chain reaction (RT-qPCR) and Western blot, respectively.
Results: The expression of miR-10b was up-regulated in non-small cell lung cancer, and miR-10b inhibitor could inhibit the proliferation of A549 cell. Meanwhile, the tumor cell-derived exosome miR-10b promoted the invasion of A549 cell and EMT by promoting the M2 polarization of macrophages.
Conclusions: Tumor cell-derived exosome miR-10b promotes A549 cell invasion and EMT through M2 macrophage polarization.
【中文题目：外泌体miR-10b通过调控巨噬细胞M2极化 促进肺腺癌A549细胞的侵袭和上皮间质转化】 【中文摘要：背景与目的 肺癌转移是引起死亡的主要原因。先天免疫细胞特别是巨噬细胞在肿瘤转移中扮演非常重要的角色。外泌体在肿瘤细胞与巨噬细胞间的通讯非常重要，因此本研究探讨肺腺癌A549细胞外泌体中的miR-10b对其细胞生长侵袭及上皮间质转化（epithelial mesenchymal transition, EMT）的影响。方法 从A549细胞中分离外泌体，通过透射电镜及蛋白质印迹法对外泌体进行鉴定。采用CCK-8试剂盒和流式细胞术检测A549细胞的增殖和凋亡。Transwell法检测细胞侵袭以及通过定量逆转录聚合酶链式反应（quantitative reverse transcription polymerase chain reaction, RT-qPCR）和Western blot分别检测A549细胞中mRNA和蛋白的表达。结果 miR-10b在非小细胞肺癌（A549、NCI-H1650和NCI-H1299）中表达上调，miR-10b inhibitor可抑制非小细胞肺癌的增殖。同时，外泌体miR-10b通过促进巨噬细胞M2极化，进而促进A549细胞的侵袭和EMT。结论 外泌体miR-10b通过M2巨噬细胞极化促进A549细胞的侵袭和EMT。 】 【中文关键词：A549；M2型巨噬细胞极化；外泌体；miR-10b；侵袭；上皮间质转化】.
Keywords: A549; Epithelial mesenchymal transition; Exosome; Invasion; M2 macrophage polarization; miR-10b.