Genes for tRNA recycling are upregulated in response to infection with Theiler's mouse encephalitis virus

Mineaki Seki; Akihiko Komuro; Tatsuya Ishikawa; Masayuki Takahashi; Masayuki Nashimoto

doi:10.1016/j.bbrc.2021.11.091

Genes for tRNA recycling are upregulated in response to infection with Theiler's mouse encephalitis virus

Biochem Biophys Res Commun. 2022 Jan 8:587:63-68. doi: 10.1016/j.bbrc.2021.11.091. Epub 2021 Nov 30.

Authors

Mineaki Seki¹, Akihiko Komuro², Tatsuya Ishikawa³, Masayuki Takahashi³, Masayuki Nashimoto³

Affiliations

¹ Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Niigata, 956-8603, Japan. Electronic address: seki@nupals.ac.jp.
² Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Niigata, 956-8603, Japan; Faculty of Pharmaceutical Sciences, Department of Biochemistry, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Niigata, 956-8603, Japan.
³ Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Niigata, 956-8603, Japan.

PMID: 34864548
DOI: 10.1016/j.bbrc.2021.11.091

Abstract

The concept of tRNA recycling has recently emerged from the studies of ribosome-associated quality control. Therein tRNase Z^S removes the 2', 3'>p from the ANKZF1-cleaved tRNA and the subsequent TRNT1 action re-generates the intact tRNA. To know the roles of the tRNA recycling in vivo, we investigated how viral infection affects the tRNA recycling system by analyzing the mRNA levels of tRNase Z^S and TRNT1. We found that both genes in HeLa cells are upregulated in response to infection of Theiler's mouse encephalitis virus but not to that of an influenza A virus. Upregulation was also observed in cells infected with encephalomyocarditis virus with reduced efficiency. The levels of the IFN-β mRNA appeared to positively correlate with those of the tRNase Z^S and TRNT1 mRNAs. The tRNase Z^S gene may be regulated post-transcriptionally in the cells infected with Theiler's mouse encephalitis virus.

Keywords: ELAC1; Infection; Interferon; Ribosome quality control; TRNT1; tRNA processing; tRNA recycling; tRNase ZS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carrier Proteins / genetics
Carrier Proteins / metabolism
Encephalomyocarditis virus / genetics
Encephalomyocarditis virus / growth & development
Encephalomyocarditis virus / metabolism
Endoribonucleases / genetics*
Endoribonucleases / metabolism
HeLa Cells
Host-Pathogen Interactions / genetics*
Humans
Influenza A virus / genetics
Influenza A virus / growth & development
Influenza A virus / metabolism
Interferon-beta / genetics
Interferon-beta / metabolism
Isoenzymes / genetics
Isoenzymes / metabolism
Nucleotidyltransferases / genetics*
Nucleotidyltransferases / metabolism
RNA Processing, Post-Transcriptional*
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Transfer / genetics*
RNA, Transfer / metabolism
Ribosomes / genetics
Ribosomes / metabolism
Theilovirus / genetics*
Theilovirus / growth & development
Theilovirus / metabolism
Viral Load

Substances

ANKZF1 protein, human
Carrier Proteins
Isoenzymes
RNA, Messenger
Interferon-beta
RNA, Transfer
Nucleotidyltransferases
TRNT1 protein, human
Endoribonucleases
tRNase Z, human