Exosomes isolated from CAPS1‑overexpressing colorectal cancer cells promote cell migration

Bingrui Wu; Dalong Sun; Lijie Ma; Yiran Deng; Si Zhang; Ling Dong; She Chen

doi:10.3892/or.2019.7361

Exosomes isolated from CAPS1‑overexpressing colorectal cancer cells promote cell migration

Oncol Rep. 2019 Dec;42(6):2528-2536. doi: 10.3892/or.2019.7361. Epub 2019 Oct 9.

Authors

Bingrui Wu¹, Dalong Sun², Lijie Ma³, Yiran Deng¹, Si Zhang¹, Ling Dong², She Chen¹

Affiliations

¹ Key Laboratory of Glycoconjugate Research (Ministry of Public Health), Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032, P.R. China.
² Department of Gastroenterology and Hepatology, Shanghai Institute of Liver Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China.
³ Department of Liver Surgery and Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Shanghai 200032, P.R. China.

Abstract

Calcium‑dependent activator protein for secretion 1 (CAPS1) has been reported to promote metastasis in colorectal cancer (CRC), however, the underlying mechanisms have not yet been elucidated. The present study revealed that exosomes derived from CAPS1‑overexpressing CRC cells could enhance the migration of normal colonic epithelial FHC cells. GW4869, an inhibitor of exosomes, could attenuate the migration of FHC cells. Furthermore, liquid chromatography‑mass spectrometry (LC‑MS) and bioinformatics analysis demonstrated that overexpression of CAPS1 could alter the expression pattern of exosomal proteins involved in cell migration. Bone morphogenetic protein 4, which may serve vital roles in the process of CAPS1‑induced cell migration, was downregulated in the exosomes. In summary, the present results demonstrated that CAPS1 promotes cell migration by regulating exosomes. Inhibiting the secretion of exosomes may be helpful for the treatment of patients with metastatic CRC.

MeSH terms

Aniline Compounds / pharmacology*
Aniline Compounds / therapeutic use
Apoptosis / drug effects
Apoptosis / genetics
Benzylidene Compounds / pharmacology*
Benzylidene Compounds / therapeutic use
Bone Morphogenetic Protein 4 / metabolism
Calcium-Binding Proteins / metabolism*
Cell Movement / drug effects
Cell Movement / genetics*
Cell Proliferation / drug effects
Cell Proliferation / genetics
Colorectal Neoplasms / drug therapy
Colorectal Neoplasms / genetics
Colorectal Neoplasms / pathology*
Computational Biology
Drug Screening Assays, Antitumor
Exosomes / drug effects
Exosomes / metabolism*
Exosomes / ultrastructure
Gene Expression Regulation, Neoplastic / drug effects
HEK293 Cells
HT29 Cells
Humans
Microscopy, Electron
Protein Interaction Mapping
Vesicular Transport Proteins / metabolism*

Substances

Aniline Compounds
BMP4 protein, human
Benzylidene Compounds
Bone Morphogenetic Protein 4
CADPS protein, human
Calcium-Binding Proteins
GW 4869
Vesicular Transport Proteins