SPTLC1 inhibits cell growth via modulating Akt/FOXO1 pathway in renal cell carcinoma cells

Biochem Biophys Res Commun. 2019 Nov 26;520(1):1-7. doi: 10.1016/j.bbrc.2019.09.073. Epub 2019 Sep 22.

Abstract

Serine palmitoyltransferase long chain-1 (SPTLC1), which is the rate-limiting enzyme for sphingolipid biosynthesis, has been indicated to be essential for carcinoma cell survival and proliferation in recent, but its role in the regulation of renal cell carcinoma (RCC) remains unknown. In the present study, we found that SPTLC1 expression was significantly decreased in RCC tissues compared to non-tumor tissues, and low SPTLC1 expression was associated with poor overall survival of RCC patients. In addition, our results revealed that forced expression of SPTLC1 could significantly inhibit cell growth in vitro and in vivo via, at least in part, modulating Akt/FOXO1 signaling pathway, thus representing a novel role of SPTLC1 in the regulation of tumor growth in RCC for the first time.

Keywords: Akt/FOXO1 pathway; Cell growth; Renal cell carcinoma; SPTLC1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carcinoma, Renal Cell / metabolism*
  • Carcinoma, Renal Cell / pathology
  • Cell Proliferation
  • Forkhead Box Protein O1 / metabolism*
  • Humans
  • Kidney Neoplasms / metabolism*
  • Kidney Neoplasms / pathology
  • Male
  • Mice
  • Mice, Nude
  • Neoplasms, Experimental / metabolism
  • Neoplasms, Experimental / pathology
  • Proto-Oncogene Proteins c-akt / metabolism*
  • Serine C-Palmitoyltransferase / biosynthesis
  • Serine C-Palmitoyltransferase / metabolism*
  • Tumor Cells, Cultured

Substances

  • FOXO1 protein, human
  • Forkhead Box Protein O1
  • SPTLC1 protein, human
  • Serine C-Palmitoyltransferase
  • Proto-Oncogene Proteins c-akt