Abstract
Muscle differentiation is a complex process in which muscle progenitor cells undergo determination and eventually cellular fusion. This process is heavily regulated by such master transcription factors as MYOD and members of the MEF2 family. Here, we show that the transcription factor ZNF148 plays a direct role in human muscle cell differentiation. Downregulation of ZNF148 drives the formation of a muscle phenotype with rapid expression of myosin heavy chain, even in proliferative conditions. This phenotype was most likely mediated by the robust and swift upregulation of MYOD and MEF2C.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Differentiation* / genetics
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Cell Line
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Cell Line, Transformed
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Gene Expression Profiling
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Gene Expression Regulation*
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Gene Knockdown Techniques
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Humans
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Muscle Development / genetics
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Muscle, Skeletal* / metabolism
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MyoD Protein / genetics
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MyoD Protein / metabolism
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Myoblasts / cytology*
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Myoblasts / metabolism*
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Phenotype
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RNA, Small Interfering / genetics
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Transcription Factors / genetics
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Transcription Factors / metabolism*
Substances
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DNA-Binding Proteins
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MyoD Protein
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RNA, Small Interfering
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Transcription Factors
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ZNF148 protein, human