Human heart cytochrome c oxidase subunit VIII. Purification and determination of the complete amino acid sequence

FEBS Lett. 1988 Nov 21;240(1-2):127-32. doi: 10.1016/0014-5793(88)80353-3.

Abstract

Subunit VIII was purified from a preparation of the human heart cytochrome c oxidase and its complete amino acid sequence was determined. The sequence proved to be much more related to that of the bovine liver oxidase subunit VIII than to that found in bovine heart. Our finding of a 'liver-type' subunit VIII in the human heart enzyme suggests that either there are no isoforms of human subunit VIII or the human oxidase does not show the type of tissue specificity that has been reported for the oxidase in other mammals.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Chromatography, High Pressure Liquid
  • Electron Transport Complex IV / genetics
  • Electron Transport Complex IV / isolation & purification*
  • Macromolecular Substances
  • Mitochondria, Heart / enzymology*
  • Molecular Sequence Data

Substances

  • Macromolecular Substances
  • Electron Transport Complex IV