Increased interleukin-1β and basic fibroblast growth factor levels in the cerebrospinal fluid during human herpesvirus-6B (HHV-6B) encephalitis

Masato Tamai; Nobuyuki Kobayashi; Kazuya Shimada; Naomi Oka; Mayumi Takahashi; Akiko Tanuma; Tomohiro Tanemoto; Hiroyuki Namba; Yoshihiro Saito; Yasuyuki Wada; Aikou Okamoto; Hiroyuki Ida; Kazuhiro Kondo

doi:10.1016/j.bbrc.2017.03.102

Increased interleukin-1β and basic fibroblast growth factor levels in the cerebrospinal fluid during human herpesvirus-6B (HHV-6B) encephalitis

Biochem Biophys Res Commun. 2017 May 6;486(3):706-711. doi: 10.1016/j.bbrc.2017.03.102. Epub 2017 Mar 22.

Authors

Affiliations

¹ Department of Virology, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan; Department of Pediatrics, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.
² Department of Virology, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan. Electronic address: kobayashi-n@jikei.ac.jp.
³ Department of Virology, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.
⁴ Department of Obstetrics and Gynecology, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.
⁵ Department of Obstetrics and Gynecology, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan; Department of General Medical Science, Chiba University Graduate School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba-shi, Chiba 260-8670, Japan.
⁶ Department of Pediatrics, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.

PMID: 28342868
DOI: 10.1016/j.bbrc.2017.03.102

Abstract

Human herpesvirus 6B (HHV-6B) causes exanthema subitum in infants and is known to be mildly pathogenic. However, HHV-6B infection can induce febrile seizures in a high percentage of patients, and in rare cases, result in encephalitis. We detected higher levels of interleukin (IL)-1β and basic fibroblast growth factor (bFGF) in the cerebrospinal fluid (CFS) of patients with HHV-6B encephalitis when compared to those in patients with non-HHV-6B-induced febrile seizures. In vitro, IL-1β and bFGF enhanced HHV-6B gene expression in infected U373 astrocytes during the initial and maintenance phases of infection, respectively. These findings indicated that IL-1β and bFGF contribute to HHV-6B growth and the onset of encephalitis.

Keywords: Astrocytes; Basic fibroblast growth factor; Encephalitis; Human herpesvirus-6; Interleukin-1β.

MeSH terms

Astrocytes / metabolism
Astrocytes / virology
Case-Control Studies
Cell Line
Child, Preschool
DNA, Viral / cerebrospinal fluid
DNA, Viral / genetics*
Encephalitis, Viral / cerebrospinal fluid
Encephalitis, Viral / genetics*
Encephalitis, Viral / pathology
Encephalitis, Viral / virology
Female
Fibroblast Growth Factors / cerebrospinal fluid
Fibroblast Growth Factors / genetics*
Gene Expression
Herpesvirus 6, Human / genetics*
Herpesvirus 6, Human / growth & development
Herpesvirus 6, Human / pathogenicity
Host-Pathogen Interactions
Humans
Infant
Interleukin-1beta / cerebrospinal fluid
Interleukin-1beta / genetics*
Male
RNA, Messenger / cerebrospinal fluid
RNA, Messenger / genetics
Seizures, Febrile / cerebrospinal fluid
Seizures, Febrile / genetics*
Seizures, Febrile / pathology
Seizures, Febrile / virology

Substances

DNA, Viral
IL1B protein, human
Interleukin-1beta
RNA, Messenger
fibroblast growth factor 13
Fibroblast Growth Factors