The association between fructosamine-3 kinase 900C/G polymorphism, transferrin polymorphism and human herpesvirus-8 infection in diabetics living in South Kivu

Acta Trop. 2016 Nov:163:14-9. doi: 10.1016/j.actatropica.2016.07.011. Epub 2016 Jul 25.

Abstract

Prevalences of human herpesvirus-8 (HHV-8) infection and diabetes mellitus are very common in certain parts of Africa, containing iron-rich soils. We hypothesized that some genetic factors could have a link with susceptibility to HHV-8 infection. We focused on ferroportin Q248H mutation (rs11568350), transferrin (TF) polymorphism and fructosamine-3 kinase (FN3K) 900C/G polymorphism (rs1056534). The study population consisted of 210 type 2 diabetic adults and 125 healthy controls recruited in Bukavu (South Kivu). In the whole study population (diabetics+healthy controls), ferroportin Q248H mutation was detected in 47 subjects (14.0%) with 43 heterozygotes and 4 homozygotes. TF phenotype frequencies were 88.1% (CC), 10.4% (CD) and 1.5% (BC). Genotype frequencies of FN3K 900C/G polymorphism were respectively 9,3% (CC), 43.3% (GC) and 47.4% (GG). Prevalence of HHV8-infection in the study population was 77.3%. HHV-8 infection rate and HHV-8 IgG antibody titer were significantly higher in diabetics then in controls (p<0.0001). Significant differences were observed in HHV-8 infection rate and in HHV-8 IgG antibody titer according to FN3K rs1056534 (p<0.05 and p<0.05, respectively) and TF polymorphism (p<0.05 and p=0.005, respectively). No significant differences in HHV-8 infection rate and in HHV-8 IgG antibody titer were observed in the ferroportin Q248H mutation carriers (rs11568350) in comparison with ferroportin wild type. In a multiple regression analysis, FN3K rs1056534, TF polymorphism and presence of diabetes mellitus were predictors for HHV-8 infection. In contrast to these findings, ferroportin Q248H mutation (rs11568350) did not influence the susceptibility for an HHV-8 infection in sub-Saharan Africans.

Keywords: Diabetes meliitus; Ferroportin Q248H mutation; Fructosamine-3 kinase 900C/G polymorphism; Human herpes virus-8; TF polymorphism.

MeSH terms

  • Adult
  • Aged
  • Case-Control Studies
  • Cation Transport Proteins / genetics
  • Democratic Republic of the Congo / epidemiology
  • Diabetes Mellitus, Type 2* / complications
  • Female
  • Genotype
  • Herpesviridae Infections / epidemiology*
  • Herpesviridae Infections / genetics
  • Herpesviridae Infections / virology
  • Herpesvirus 8, Human*
  • Humans
  • Male
  • Middle Aged
  • Phosphotransferases (Alcohol Group Acceptor) / genetics*
  • Polymorphism, Genetic*
  • Prevalence
  • Regression Analysis
  • Transferrin / genetics*

Substances

  • Cation Transport Proteins
  • Transferrin
  • metal transporting protein 1
  • Phosphotransferases (Alcohol Group Acceptor)
  • fructosamine-3-kinase