Implication of JAK1, JAK2, and JAK3 in the Realization of Proliferation and Differentiation Potential of Mesenchymal Progenitor Cells In Vitro

G N Zyuz'kov; V V Zhdanov; E V Udut; L A Miroshnichenko; E V Simanina; T Yu Polyakova; A V Chaikovskii; L A Stavrova; V V Udut; V I Agafonov; Ya V Burmina; M G Danilets; M Yu Minakova; A M Dygai

doi:10.1007/s10517-016-3381-7

Implication of JAK1, JAK2, and JAK3 in the Realization of Proliferation and Differentiation Potential of Mesenchymal Progenitor Cells In Vitro

Bull Exp Biol Med. 2016 Jun;161(2):224-7. doi: 10.1007/s10517-016-3381-7. Epub 2016 Jul 7.

Authors

G N Zyuz'kov^{1

2}, V V Zhdanov³, E V Udut³, L A Miroshnichenko³, E V Simanina³, T Yu Polyakova³, A V Chaikovskii³, L A Stavrova³, V V Udut^{3

4}, V I Agafonov³, Ya V Burmina³, M G Danilets³, M Yu Minakova³, A M Dygai³

Affiliations

¹ E.D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia. zgn@pharm.tsu.ru.
² National Research Tomsk State University, Tomsk, Russia. zgn@pharm.tsu.ru.
³ E.D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
⁴ National Research Tomsk State University, Tomsk, Russia.

PMID: 27383175
DOI: 10.1007/s10517-016-3381-7

Abstract

Involvement of individual JAK kinases in the realization of growth potential of mesenchymal progenitor cells was examined in vitro. Important role of JAK2 and JAK3 in determining the initial level of mitotic activity of progenitor cells was established. The yield of fibroblast CFUF was suppressed under the effect of specific inhibitors of JAK kinases. Blockade of JAK3 increased the rate of progenitor element differentiation. JAK1 had no effect on proliferation and differentiation status of progenitor cells.

Keywords: JAK kinases; pharmacological control; progenitor cells; regenerative medicine; signal pathways.

MeSH terms

Animals
Cell Differentiation*
Cell Proliferation*
Cells, Cultured
Janus Kinases / physiology*
MAP Kinase Signaling System
Male
Mesenchymal Stem Cells / enzymology*
Mice, Inbred CBA

Substances

Janus Kinases