Abstract
Competing endogenous RNAs (ceRNAs) network has been correlated with the initiation and development of cancer. Here, we identify CDH5, HOXD1, and HOXD10 as putative STARD13 ceRNAs and they display concordant patterns with STARD13 in different metastatic potential breast cancer cell lines and tissues. Notably, 3'UTRs of these genes suppress breast cancer metastasis via inhibiting epithelial-mesenchymal transition (EMT) in vitro and in vivo, which are activated through the crosstalk between STARD13 and its ceRNAs in 3'UTR- and miRNA-dependent manners. In addition, Kaplan-Meier survival analysis reveals that mRNA level of STARD13 and its ceRNAs is remarkably associated with survival of breast cancer patients. These results suggest that 3'UTRs of CDH5, HOXD1, and HOXD10 inhibit breast cancer metastasis via serving as STARD13 ceRNAs.
Keywords:
3′UTRs; EMT; STARD13; breast cancer metastasis; ceRNA.
MeSH terms
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3' Untranslated Regions
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Animals
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Apoptosis
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Biomarkers, Tumor / genetics
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Breast Neoplasms / genetics
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Breast Neoplasms / metabolism
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Breast Neoplasms / pathology*
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Cell Proliferation
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Epithelial-Mesenchymal Transition*
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Female
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GTPase-Activating Proteins
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Gene Expression Regulation, Neoplastic*
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Humans
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Liver Neoplasms / genetics
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Liver Neoplasms / metabolism
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Liver Neoplasms / secondary*
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Lung Neoplasms / genetics
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Lung Neoplasms / metabolism
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Lung Neoplasms / secondary*
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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MicroRNAs / genetics
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Prognosis
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RNA, Long Noncoding / genetics*
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RNA, Messenger
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Survival Rate
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Tumor Cells, Cultured
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Tumor Suppressor Proteins / genetics
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Tumor Suppressor Proteins / metabolism*
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Xenograft Model Antitumor Assays
Substances
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3' Untranslated Regions
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Biomarkers, Tumor
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GTPase-Activating Proteins
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MicroRNAs
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RNA, Long Noncoding
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RNA, Messenger
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STARD13 protein, human
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Tumor Suppressor Proteins