[The number of peripheral blood CD11c+ antigen presenting cells increases and their function strengthens in the patients with active pulmonary tuberculosis]

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016 Mar;32(3):378-81.
[Article in Chinese]

Abstract

Objective: To detect the percentage of CD11c positive antigen presenting cells (CD11c(+) APCs) in peripheral blood from patients with active pulmonary tuberculosis (APT) and the levels of HLA-DR and CD86. Methods Fifty-two APT patients were enrolled in the study and 15 healthy volunteers served as controls. The frequencies of CD11c(+) APCs and the expressions of HLA-DR and CD86 in CD11c(+) APCs in the peripheral blood were determined by flow cytometry.

Results: The percentage of CD11c(+) APCs in the peripheral blood in the patients with APT was much higher than that in the controls. Interestingly, CD11c(+) APCs frequency in post-treatment patients was even higher compared with that in the pre-treatment patients. Furthermore, both HLA-DR(+) CD11c(+) APC frequency and the mean fluorescence intensity (MFI) of HLA-DR in APT patients were higher than those in the controls. Similarly, the percentage of CD86(+) CD11c(+) APCs in the APT patients was also higher than that in the controls.

Conclusion: The increase of CD11c(+) APCs with high levels of HLA-DR and CD86 in APT patients suggests that the antigen presenting capacity of APCs is at a high level in APT patients.

MeSH terms

  • Adolescent
  • Adult
  • Antigen Presentation / drug effects
  • Antigen Presentation / immunology
  • Antigen-Presenting Cells / immunology*
  • Antigen-Presenting Cells / metabolism
  • Antitubercular Agents / therapeutic use
  • B7-2 Antigen / blood
  • B7-2 Antigen / immunology
  • CD11c Antigen / blood
  • CD11c Antigen / immunology*
  • Cell Count
  • Female
  • Flow Cytometry
  • HLA-DR Antigens / blood
  • HLA-DR Antigens / immunology
  • Host-Pathogen Interactions / drug effects
  • Host-Pathogen Interactions / immunology
  • Humans
  • Male
  • Middle Aged
  • Mycobacterium tuberculosis / drug effects
  • Mycobacterium tuberculosis / immunology*
  • Mycobacterium tuberculosis / physiology
  • Treatment Outcome
  • Tuberculosis, Pulmonary / blood
  • Tuberculosis, Pulmonary / drug therapy
  • Tuberculosis, Pulmonary / immunology*
  • Young Adult

Substances

  • Antitubercular Agents
  • B7-2 Antigen
  • CD11c Antigen
  • HLA-DR Antigens