Determinants of Human CD134 Essential for Entry of Human Herpesvirus 6B

Huamin Tang; Yasuko Mori

doi:10.1128/JVI.01606-15

Determinants of Human CD134 Essential for Entry of Human Herpesvirus 6B

J Virol. 2015 Oct;89(19):10125-9. doi: 10.1128/JVI.01606-15. Epub 2015 Jul 22.

Authors

Huamin Tang¹, Yasuko Mori²

Affiliations

¹ Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, Japan Department of Immunology, Nanjing Medical University, Nanjing, China.
² Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, Japan ymori@med.kobe-u.ac.jp.

Abstract

We identified two key amino acid residues within human CD134 (hCD134) that are required for its interaction with human herpesvirus 6B (HHV-6B) and for HHV-6B entry into cells. One of the residues (K79) allows access of the HHV-6B ligand to hCD134. Murine CD134 (mCD134) functioned as an HHV-6B receptor when these two amino acid residues were replaced with homologous human residues. This study identifies both the HHV-6B receptor-ligand interaction and the species-specific determinants of hCD134 essential for HHV-6B entry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Amino Acid Substitution
Animals
Cell Line
Crystallography, X-Ray
Herpesvirus 6, Human / pathogenicity
Herpesvirus 6, Human / physiology*
Host-Pathogen Interactions / genetics
Host-Pathogen Interactions / physiology
Humans
Ligands
Mice
Models, Molecular
Molecular Sequence Data
Mutagenesis, Site-Directed
Protein Conformation
Receptors, OX40 / chemistry*
Receptors, OX40 / genetics
Receptors, OX40 / physiology*
Receptors, Virus / chemistry
Receptors, Virus / genetics
Receptors, Virus / physiology
Sequence Homology, Amino Acid
Virus Internalization*

Substances

Ligands
Receptors, OX40
Receptors, Virus
TNFRSF4 protein, human
Tnfrsf4 protein, mouse

Associated data

PDB/2HEV