Human gallbladder cancer is a rare malignancy disease but having poor prognosis over the world. Previous studies have put forward that PTEN is a tumour suppressor in regulating many cellular processes, similar activities have been observed for its mammal homologue TPTE2. In this study, we attempted to unravel the underlying mechanistic basis of the role of TPTE2 and its pseudogene TPTE2P1 in gallbladder cancer. We employed lentivirus-mediated RNA interference as an efficient tool to silence endogenous TPTE2P1 transcription in the gallbladder cancer cell line GBC-SD/M. The effects of TPTE2P1 on cell migration and invasion were determined by transwell assays. We figured that depletion of TPTE2P1 remarkably inhibited gallbladder cancer cell migration and invasion capacity in vitro and elevated the expression of β-catenin via epithelial-mesenchymal transition signalling. Our findings revealed the functional role of TPTE2P1 in human gallbladder cancer and suggested that TPTE2P1 could serve as a promising therapeutic target and a palliation option in human gallbladder cancer.
Keywords: RNA interference; TPTE2P1; cell migration; gallbladder cancer; β-catenin.
© 2015 John Wiley & Sons A/S.