Heterozygous Igf1r deletion does not ameliorate pathological features associated with polyglutamine-containing huntingtin fragment

Neurosci Lett. 2014 Sep 19:580:52-5. doi: 10.1016/j.neulet.2014.07.049. Epub 2014 Aug 4.

Abstract

The evolutionarily conserved insulin/IGF-1 signaling pathway has pleiotropic effects on various cellular processes. Hypomorphic alleles of the insulin/IGF-1 receptor enhance catabolic processes as well as stress resistance, which ultimately lead to lifespan extension in invertebrates. Moreover, decreased insulin/IGF-1 signaling promotes the maintenance of protein quality control and suppresses the onset of cellular toxicity caused by aggregate-prone proteins. As loss of protein homeostasis is a feature of many sporadic and inherited forms of neurodegenerative disorders, the pharmacological inhibition of the IGF-1 receptor represents a promising potential therapeutic strategy for currently untreatable neurodegenerative disorders. However, additional studies are required to determine whether this approach is suitable to delay pathology in clinically relevant models of neurodegenerative disorders. Here we show that, in a mouse model of Huntington's disease, heterozygous knockout of the Igf1r does not prevent premature lethality of mice expressing a short fragment of the mutant human huntingtin. Moreover, Igf1r haploinsufficiency does not suppress the formation of huntingtin-containing aggregates. Thus, partial genetic manipulation of the insulin/IGF-1 signaling pathway does not seem sufficient to counteract protein toxicity and extend animal survival.

Keywords: Huntington's disease; Igf1r; Intracellular aggregates; PI3K/Akt signaling pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Glutamine / analogs & derivatives
  • Glutamine / metabolism
  • Heterozygote
  • Humans
  • Huntingtin Protein
  • Huntington Disease / genetics
  • Huntington Disease / pathology*
  • Mice, Knockout
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / metabolism
  • Peptides / metabolism
  • Protein Aggregates
  • Receptor, IGF Type 1 / genetics*
  • Receptor, IGF Type 1 / metabolism

Substances

  • HTT protein, human
  • Huntingtin Protein
  • Nerve Tissue Proteins
  • Peptides
  • Protein Aggregates
  • Glutamine
  • polyglutamine
  • Receptor, IGF Type 1