Proteomics-based identification of the tumor suppressor role of aminoacylase 1 in hepatocellular carcinoma

Xuyong Wei; Jie Li; Haiyang Xie; Qi Ling; Jianguo Wang; Di Lu; Lin Zhou; Xiao Xu; Shusen Zheng

doi:10.1016/j.canlet.2014.05.001

Proteomics-based identification of the tumor suppressor role of aminoacylase 1 in hepatocellular carcinoma

Cancer Lett. 2014 Aug 28;351(1):117-25. doi: 10.1016/j.canlet.2014.05.001. Epub 2014 May 15.

Authors

Xuyong Wei¹, Jie Li¹, Haiyang Xie¹, Qi Ling², Jianguo Wang¹, Di Lu¹, Lin Zhou¹, Xiao Xu³, Shusen Zheng⁴

Affiliations

¹ Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, Hangzhou 310003, China.
² Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
³ Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China. Electronic address: zjxu@zju.edu.cn.
⁴ Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China. Electronic address: zyzss@zju.edu.cn.

PMID: 24846301
DOI: 10.1016/j.canlet.2014.05.001

Abstract

The present work aimed to investigate the expression and role of aminoacylase 1 (ACY1) in hepatocellular carcinoma (HCC) based on a proteomic study. The study results revealed that the expression of ACY1 was much lower in HCC tissues. ACY1 expression significantly correlated with the serum alpha fetoprotein level and tumor invasiveness. The knockdown of ACY1 in SMMC7721 cells promoted cell viability and invasiveness. In contrast, the restoration of ACY1 in BEL7402 cells inhibited cell viability and invasiveness. Further studies indicated that the knockdown of ACY1 increased the protein expression of transforming growth factor beta 1 and extracellular signal-regulated kinase 1 expression. The study's results indicated that ACY1 acts as a tumor suppressor in HCC.

Keywords: Aminoacylase 1; Hepatocellular carcinoma; Proteomic; Tumor suppressor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amidohydrolases / physiology*
Apoptosis
Carcinoma, Hepatocellular / enzymology*
Female
Gene Knockdown Techniques
Hep G2 Cells
Humans
Liver Neoplasms / enzymology*
Male
Middle Aged
Proteome / metabolism*
Proteomics
S Phase Cell Cycle Checkpoints
Tandem Mass Spectrometry
Tumor Suppressor Proteins / physiology*
Two-Dimensional Difference Gel Electrophoresis

Substances

Proteome
Tumor Suppressor Proteins
Amidohydrolases
aminoacylase I