Methylation of SFRPs and APC genes in ovarian cancer infected with high risk human papillomavirus

Asian Pac J Cancer Prev. 2014;15(6):2719-25. doi: 10.7314/apjcp.2014.15.6.2719.

Abstract

Background: Secreted frizzled-related protein (SFRP) genes, new tumor suppressor genes, are negative regulators of the Wnt pathway whose alteration is associated with various tumors. In ovarian cancer, SFRPs genes promoter methylation can lead to gene inactivation. This study investigated mechanisms of SFRP and adenomatous polyposis coli (APC) genes silencing in ovarian cancer infected with high risk human papillomavirus.

Materials and methods: DNA was extracted from 200 formalin-fixed paraffin-embedded ovarian cancer and their normal adjacent tissues (NAT) and DNA methylation was detected by methylation specific PCR (MSP). High risk human papillomavirus (HPV) was detected by nested PCR with consensus primers to amplify a broad spectrum of HPV genotypes.

Results: The percentages of SFRP and APC genes with methylation were significantly higher in ovarian cancer tissues infected with high risk HPV compared to NAT. The methylated studied genes were associated with suppression in their gene expression.

Conclusion: This finding highlights the possible role of the high risk HPV virus in ovarian carcinogenesis or in facilitating cancer progression by suppression of SFRP and APC genes via DNA methylation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenomatous Polyposis Coli Protein / genetics*
  • Adult
  • Aged
  • Aged, 80 and over
  • DNA Methylation*
  • Female
  • Follow-Up Studies
  • Gene Expression Regulation, Neoplastic
  • Glycoproteins / genetics*
  • Humans
  • Intercellular Signaling Peptides and Proteins / genetics*
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins / genetics*
  • Middle Aged
  • Neoplasm Staging
  • Ovarian Neoplasms / genetics*
  • Ovarian Neoplasms / pathology
  • Ovarian Neoplasms / virology
  • Papillomaviridae / classification
  • Papillomaviridae / genetics
  • Papillomavirus Infections / genetics*
  • Papillomavirus Infections / pathology
  • Papillomavirus Infections / virology
  • Prognosis
  • Promoter Regions, Genetic
  • Proto-Oncogene Proteins / genetics*
  • Risk Factors
  • Young Adult

Substances

  • Adenomatous Polyposis Coli Protein
  • Glycoproteins
  • Intercellular Signaling Peptides and Proteins
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Proto-Oncogene Proteins
  • SFRP1 protein, human
  • SFRP2 protein, human
  • SFRP4 protein, human
  • WD repeat containing planar cell polarity effector