Interaction with the Yes-associated protein (YAP) allows TEAD1 to positively regulate NAIP expression

FEBS Lett. 2013 Oct 1;587(19):3216-23. doi: 10.1016/j.febslet.2013.08.013. Epub 2013 Aug 28.

Abstract

Although the expression of the neuronal apoptosis inhibitory protein (NAIP) gene is considered involved in apoptosis suppression as well as in inflammatory response, the molecular basis of the NAIP gene expression is poorly understood. Here we show that the TEA domain protein 1 (TEAD1) is able to positively activate the transcription of NAIP. We further demonstrate that this regulation is mediated by the presence of the endogenous Yes associated protein (YAP) cofactor, and requires the interaction with YAP. We finally identified an intronic region of the NAIP gene responding to TEAD1/YAP activity, suggesting that regulation of NAIP by TEAD1/YAP is at the transcriptional level.

Keywords: Hippo pathway; IAP; LATS; MST; NAIP; TEA domain; TEAD; TEAD1; YAP; Yes-associated protein; Yki; Yorkie; inhibitor of apoptosis protein; large tumour suppressor; mammalian Ste20-like protein kinase; neuronal apoptosis-inhibitory protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / metabolism*
  • Base Sequence
  • Cell Line, Tumor
  • DNA
  • DNA-Binding Proteins / metabolism*
  • DNA-Binding Proteins / physiology
  • Gene Expression Regulation*
  • Humans
  • Molecular Sequence Data
  • Neuronal Apoptosis-Inhibitory Protein / genetics*
  • Nuclear Proteins / metabolism*
  • Nuclear Proteins / physiology
  • Phosphoproteins / metabolism*
  • Protein Binding
  • TEA Domain Transcription Factors
  • Transcription Factors / metabolism*
  • Transcription Factors / physiology
  • Transcription, Genetic
  • YAP-Signaling Proteins

Substances

  • Adaptor Proteins, Signal Transducing
  • DNA-Binding Proteins
  • NAIP protein, human
  • Neuronal Apoptosis-Inhibitory Protein
  • Nuclear Proteins
  • Phosphoproteins
  • TEA Domain Transcription Factors
  • TEAD1 protein, human
  • Transcription Factors
  • YAP-Signaling Proteins
  • YAP1 protein, human
  • DNA