The role of hnRPUL1 involved in DNA damage response is related to PARP1

Zehui Hong; Jie Jiang; Jiaolong Ma; Shikui Dai; Ting Xu; Hui Li; Akira Yasui

doi:10.1371/journal.pone.0060208

The role of hnRPUL1 involved in DNA damage response is related to PARP1

PLoS One. 2013;8(4):e60208. doi: 10.1371/journal.pone.0060208. Epub 2013 Apr 5.

Authors

Zehui Hong¹, Jie Jiang, Jiaolong Ma, Shikui Dai, Ting Xu, Hui Li, Akira Yasui

Affiliation

¹ Department of Genetics and Developmental Biology, Medical School of Southeast University, The Key Laboratory of Developmental Genes and Human Disease in Ministry of Education, Nanjing, China. hongzehui@seu.edu.cn

Abstract

Heterogeneous nuclear ribonucleoprotein U-like 1 (hnRPUL1) -also known as adenovirus early region 1B-associated proteins 5 (E1B-AP5) - plays a role in RNA metabolism. Recently, hnRPUL1 has also been shown to be involved in DNA damage response, but the function of hnRPUL1 in response to DNA damage remains unclear. Here, we have demonstrated that hnRPUL1 is associated with PARP1 and recruited to DNA double-strand breaks (DSBs) sites in a PARP1-mediated poly (ADP-ribosyl) ation dependent manner. In turn, hnRPUL1 knockdown enhances the recruitment of PARP1 to DSBs sites. Specifically, we showed that hnRPUL1 is also implicated in the transcriptional regulation of PARP1 gene. Thus, we propose hnRPUL1 as a new component related to PARP1 in DNA damage response and repair.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Diphosphate / metabolism
Animals
Cell Line
DNA Breaks, Double-Stranded
DNA Damage*
Down-Regulation / genetics
Genomic Instability
Heterogeneous-Nuclear Ribonucleoproteins / chemistry
Heterogeneous-Nuclear Ribonucleoproteins / metabolism*
Humans
Mice
Nuclear Proteins / chemistry
Nuclear Proteins / metabolism*
Poly (ADP-Ribose) Polymerase-1
Poly(ADP-ribose) Polymerases / genetics
Poly(ADP-ribose) Polymerases / metabolism*
Protein Transport / genetics
Ribose / metabolism
Transcription Factors / chemistry
Transcription Factors / metabolism*
Transcription, Genetic / genetics

Substances

HNRNPUL1 protein, human
Heterogeneous-Nuclear Ribonucleoproteins
Nuclear Proteins
Transcription Factors
Adenosine Diphosphate
Ribose
PARP1 protein, human
Poly (ADP-Ribose) Polymerase-1
Poly(ADP-ribose) Polymerases

Grants and funding

This work was supported by National Natural Science Foundation of China (90919007 and 30900812), New-Century Training Program Foundation for the Talents by the State Education Commission and Youth Foundation of southeast university to ZH; and the Ministry of Education, Culture, Sports, Science and Technology, Japan to AY (20241011 and 22131005). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.