Gas2l3, a novel constriction site-associated protein whose regulation is mediated by the APC/C Cdh1 complex

PLoS One. 2013;8(2):e57532. doi: 10.1371/journal.pone.0057532. Epub 2013 Feb 28.

Abstract

Growth arrest-specific 2-like protein 3 (Gas2l3) was recently identified as an Actin/Tubulin cross-linker protein that regulates cytokinesis. Using cell-free systems from both frog eggs and human cells, we show that the Gas2l3 protein is targeted for ubiquitin-mediated proteolysis by the APC/C(Cdh1) complex, but not by the APC/C(Cdc20) complex, and is phosphorylated by Cdk1 in mitosis. Moreover, late in cytokinesis, Gas2l3 is exclusively localized to the constriction sites, which are the narrowest parts of the intercellular bridge connecting the two daughter cells. Overexpression of Gas2l3 specifically interferes with cell abscission, which is the final stage of cell division, when the cutting of the intercellular bridge at the constriction sites occurs. We therefore suggest that Gas2l3 is part of the cellular mechanism that terminates cell division.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism*
  • Amino Acid Sequence
  • Animals
  • Antigens, CD
  • Cadherins / metabolism*
  • Cell Cycle
  • Humans
  • Microfilament Proteins / chemistry
  • Microfilament Proteins / metabolism*
  • Microtubule-Associated Proteins / chemistry
  • Microtubule-Associated Proteins / metabolism*
  • Molecular Sequence Data
  • Tubulin / metabolism*
  • Xenopus laevis

Substances

  • Actins
  • Antigens, CD
  • CDH1 protein, human
  • Cadherins
  • Gas2l3 protein, human
  • Microfilament Proteins
  • Microtubule-Associated Proteins
  • Tubulin