Role of leukotriene B₄ receptor signaling in human preadipocyte differentiation

Biochem Biophys Res Commun. 2012 Dec 14;429(3-4):197-203. doi: 10.1016/j.bbrc.2012.10.110. Epub 2012 Nov 5.

Abstract

We investigated the role of leukotriene B(4) (LTB(4))-leukotriene receptor (BLT) signaling in preadipocyte differentiation into mature adipocytes. Blockade of BLT signaling by treatment with lipoxygenase inhibitors, a BLT antagonist, and small interfering RNAs for BLTs in human and mouse preadipocytes isolated from adipose tissues showed acceleration of differentiation into mature adipocytes. DNA microarray analysis revealed regulation of transforming growth factor, beta-induced 68 kDa (TGFBI) expression through the BLT signaling pathway during adipocyte differentiation. Knockdown of TGFBI also showed acceleration of preadipocyte differentiation. The LTB(4)-BLT signaling pathway may negatively regulate preadipocyte differentiation via induction of TGFBI expression as a rate-limiting system to control adipocyte differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / cytology*
  • Adipocytes / drug effects
  • Adipocytes / metabolism
  • Adipogenesis / drug effects
  • Adipogenesis / genetics
  • Adipogenesis / physiology*
  • Animals
  • Cells, Cultured
  • Extracellular Matrix Proteins / biosynthesis
  • Extracellular Matrix Proteins / genetics
  • Gene Knockdown Techniques
  • Humans
  • Lipoxygenase Inhibitors / pharmacology
  • Mice
  • RNA, Small Interfering / genetics
  • Receptors, Leukotriene B4 / antagonists & inhibitors
  • Receptors, Leukotriene B4 / genetics
  • Receptors, Leukotriene B4 / physiology*
  • Transforming Growth Factor beta / biosynthesis
  • Transforming Growth Factor beta / genetics

Substances

  • Extracellular Matrix Proteins
  • LTB4R protein, human
  • LTB4R2 protein, human
  • Lipoxygenase Inhibitors
  • RNA, Small Interfering
  • Receptors, Leukotriene B4
  • Transforming Growth Factor beta
  • betaIG-H3 protein