Human family with sequence similarity 60 member A (FAM60A) protein: a new subunit of the Sin3 deacetylase complex

Mol Cell Proteomics. 2012 Dec;11(12):1815-28. doi: 10.1074/mcp.M112.020255. Epub 2012 Sep 14.

Abstract

Here we describe the function of a previously uncharacterized protein, named family with sequence similarity 60 member A (FAM60A) that maps to chromosome 12p11 in humans. We use quantitative proteomics to determine that the main biochemical partners of FAM60A are subunits of the Sin3 deacetylase complex and show that FAM60A resides in active HDAC complexes. In addition, we conduct gene expression pathway analysis and find that FAM60A regulates expression of genes that encode components of the TGF-beta signaling pathway. Moreover, our studies reveal that loss of FAM60A or another component of the Sin3 complex, SDS3, leads to a change in cell morphology and an increase in cell migration. These studies reveal the function of a previously uncharacterized protein and implicate the Sin3 complex in suppressing cell migration.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cell Movement / genetics*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Gene Expression Profiling
  • Gene Expression Regulation*
  • HEK293 Cells
  • Hep G2 Cells
  • Histone Deacetylase Inhibitors / metabolism
  • Homeodomain Proteins / genetics
  • Humans
  • Protein Structure, Tertiary
  • RNA Interference
  • RNA, Small Interfering
  • Receptors, Cytoplasmic and Nuclear / genetics
  • Repressor Proteins / metabolism*
  • Sequence Analysis, Protein
  • Sin3 Histone Deacetylase and Corepressor Complex / analysis
  • Sin3 Histone Deacetylase and Corepressor Complex / genetics
  • Sin3 Histone Deacetylase and Corepressor Complex / metabolism*
  • Transforming Growth Factor beta / genetics
  • Transforming Growth Factor beta / metabolism
  • Tumor Suppressor Proteins / genetics

Substances

  • DNA-Binding Proteins
  • Histone Deacetylase Inhibitors
  • Homeodomain Proteins
  • ING2 protein, human
  • RNA, Small Interfering
  • Receptors, Cytoplasmic and Nuclear
  • Repressor Proteins
  • SINHCAF protein, human
  • SUDS3 protein, human
  • Transforming Growth Factor beta
  • Tumor Suppressor Proteins
  • Sin3 Histone Deacetylase and Corepressor Complex