Competitive binding of UBPY and ubiquitin to the STAM2 SH3 domain revealed by NMR

Anja Lange; Mouhamad-Baligh Ismail; Gwladys Rivière; Maggy Hologne; Denis Lacabanne; Florence Guillière; Jean-Marc Lancelin; Isabelle Krimm; Olivier Walker

doi:10.1016/j.febslet.2012.07.047

Competitive binding of UBPY and ubiquitin to the STAM2 SH3 domain revealed by NMR

FEBS Lett. 2012 Sep 21;586(19):3379-84. doi: 10.1016/j.febslet.2012.07.047. Epub 2012 Jul 25.

Authors

Anja Lange¹, Mouhamad-Baligh Ismail, Gwladys Rivière, Maggy Hologne, Denis Lacabanne, Florence Guillière, Jean-Marc Lancelin, Isabelle Krimm, Olivier Walker

Affiliation

¹ Université de Lyon, Université Claude Bernard Lyon1, CNRS, UMR 5280 Institut des Sciences Analytiques, Villeurbanne, France.

PMID: 22841719
DOI: 10.1016/j.febslet.2012.07.047

Abstract

To date, the signal transducing adaptor molecule 2 (STAM2) was shown to harbour two ubiquitin binding domains (UBDs) known as the VHS and UIM domains, while the SH3 domain of STAM2 was reported to interact with deubiquitinating enzymes (DUBs) like UBPY and AMSH. In the present study, NMR evidences the interaction of the STAM2 SH3 domain with ubiquitin, demonstrating that SH3 constitutes the third UBD of STAM2. Furthermore, we show that a UBPY-derived peptide can outcompete ubiquitin for SH3 binding and vice versa. These results suggest that the SH3 domain of STAM2 plays versatile roles in the context of ubiquitin mediated receptor sorting.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptor Proteins, Signal Transducing / chemistry*
Adaptor Proteins, Signal Transducing / genetics
Adaptor Proteins, Signal Transducing / metabolism*
Amino Acid Sequence
Amino Acid Substitution
Binding, Competitive
Endopeptidases / chemistry
Endopeptidases / metabolism*
Endosomal Sorting Complexes Required for Transport / chemistry*
Endosomal Sorting Complexes Required for Transport / genetics
Endosomal Sorting Complexes Required for Transport / metabolism*
Humans
Models, Molecular
Molecular Dynamics Simulation
Molecular Sequence Data
Mutagenesis, Site-Directed
Nuclear Magnetic Resonance, Biomolecular
Protein Interaction Domains and Motifs
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Sequence Homology, Amino Acid
Ubiquitin / chemistry
Ubiquitin / metabolism*
Ubiquitin Thiolesterase / chemistry
Ubiquitin Thiolesterase / metabolism*
src Homology Domains

Substances

Adaptor Proteins, Signal Transducing
Endosomal Sorting Complexes Required for Transport
Recombinant Proteins
STAM2 protein, human
Ubiquitin
Endopeptidases
USP8 protein, human
Ubiquitin Thiolesterase