Molecular cloning and expression of an IL-6 signal transducer, gp130

Cell. 1990 Dec 21;63(6):1149-57. doi: 10.1016/0092-8674(90)90411-7.

Abstract

Interleukin-6 (IL-6) signal is transduced through a membrane glycoprotein, gp130, which associates with IL-6 receptor (IL-6-R). A cDNA encoding human gp130 has been cloned, revealing that it consists of 918 amino acids with a single transmembrane domain. The extracellular region comprises six units of a fibronectin type III module, and part of this region of approximately 200 amino acids has features typical of a cytokine receptor family. A cDNA-expressed gp130 showed no binding property to IL-6 or several other cytokines. Although a transfectant with an IL-6-R cDNA expressed mainly low affinity IL-6 binding sites, an increase in high affinity binding sites was observed after cotransfection with a gp130 cDNA. This confirmed that a gp130 is involved in the formation of high affinity IL-6 binding sites. A cloned gp130 could associate with a complex of IL-6 and soluble IL-6-R and transduce the growth signal when expressed in a murine IL-3-dependent cell line.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / metabolism
  • Antigens, CD*
  • Base Sequence
  • Cell Division
  • Cell Line
  • Cloning, Molecular / methods
  • Cytokine Receptor gp130
  • Female
  • Gene Expression
  • Gene Library
  • Humans
  • Interleukin-6 / metabolism
  • Interleukin-6 / physiology*
  • Membrane Glycoproteins / genetics*
  • Molecular Sequence Data
  • Placenta / metabolism
  • Pregnancy
  • Receptors, Immunologic / genetics*
  • Receptors, Immunologic / immunology
  • Receptors, Immunologic / physiology
  • Receptors, Interleukin-6
  • Recombinant Proteins / metabolism
  • Sequence Homology, Nucleic Acid
  • Signal Transduction
  • Transfection

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • IL6ST protein, human
  • Interleukin-6
  • Membrane Glycoproteins
  • Receptors, Immunologic
  • Receptors, Interleukin-6
  • Recombinant Proteins
  • Cytokine Receptor gp130

Associated data

  • GENBANK/M57230