Expression of a murine homolog of apoptosis-inducing human IL-24/MDA-7 in murine tumors fails to induce apoptosis or produce anti-tumor effects

Cell Immunol. 2012 Jan-Feb;275(1-2):90-7. doi: 10.1016/j.cellimm.2012.02.010. Epub 2012 Mar 14.

Abstract

Expression of human interleukin (IL)-24 in tumors achieved anti-tumor effects through apoptosis. IL-24 also induced secretion of proinflammatory cytokines, suggesting the role in immunity. We showed that murine IL-24 transcripts started from the second initiation codon and that expressed mIL-24 in tumors failed to induce apoptosis. Proliferation of murine cells expressing mIL-24 was the same as that of the parent cells and inoculation of the mIL-24-expressing tumors into syngeneic mice did not produce anti-tumor effects. Secretory mIL-24 did not induce the expression of the IL-6, TNF-α or IFN-γ gene in spleen cells. Expression of mIL-24 receptor subunits, IL-22R and IL-20R1, was undetectable in spleen cells even though they were stimulated by anti-CD3, anti-CD40 antibody or concanavalin A. Transduction of murine tumors with adenoviruses expressing the human IL-24 gene however suppressed the viability and decreased the tumor growth. These data suggest that mIL-24 is functionally irrelevant to the human counterpart.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Apoptosis*
  • Cell Line
  • Cytokines / chemistry
  • Cytokines / genetics
  • Cytokines / immunology*
  • Female
  • Gene Expression Regulation*
  • Humans
  • Interleukins / genetics
  • Interleukins / immunology*
  • Mice
  • Molecular Sequence Data
  • Neoplasms / genetics
  • Neoplasms / immunology*
  • Neoplasms / pathology
  • Sequence Alignment

Substances

  • Cytokines
  • Il24 protein, mouse
  • Interleukins
  • interleukin-24