The human ubiquitin C promoter drives selective expression in principal neurons in the brain of a transgenic mouse line

Neurochem Int. 2011 Nov;59(6):976-80. doi: 10.1016/j.neuint.2011.07.008. Epub 2011 Jul 23.

Abstract

The specificity of promoters used to drive the expression of proteins of interest is a crucial determinant of transgenesis. Numerous strategies have been developed to restrict expression on a certain cell population. On the other hand it has also remained challenging to obtain ubiquitous expression of transgenes which is needed for example to generate recombination reporter mice or to induce expression by recombination mediated excision of STOP-cassettes. We have generated transgenic mice with the expression of nuclear β-galactosidase driven by the human ubiquitin C promoter thought to mediate ubiquitous expression. However, in the brains of these transgenic mice the expression of the transgene was strikingly limited to principal neurons, while no expression was detected in interneurons or glial cells. These results indicate that the human ubiquitin C promoter might be useful to selectively target projections neurons of the brain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / cytology
  • Brain / metabolism*
  • Female
  • Gene Expression Regulation / genetics*
  • Genes, Reporter / physiology
  • Humans
  • Lac Operon / physiology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Molecular Biology / methods
  • Neural Pathways / cytology
  • Neural Pathways / metabolism
  • Neuroanatomical Tract-Tracing Techniques / methods*
  • Neuronal Tract-Tracers / metabolism
  • Neurons / cytology
  • Neurons / metabolism*
  • Promoter Regions, Genetic / genetics*
  • Transfection / methods
  • Transgenes / physiology
  • Ubiquitin C / genetics*
  • Ubiquitin C / metabolism*
  • Ubiquitination / genetics

Substances

  • Neuronal Tract-Tracers
  • Ubiquitin C