Abstract
This study was designed to investigate the expression of four actin-binding proteins, alpha-actinin-4, cofilin 1, fascin and elongation factor 1-alpha 2 (eEF1A2), in samples of breast cancer from 112 patients with different stages of breast cancer (stages T0 - T1, T2 and T3) compared with normal control tissues (n = 33). Levels of eEF1A2 and alpha-actinin-4 mRNA appeared to be unrelated to tumour size, except for a significant down-regulation of alpha-actinin-4 mRNA in T3 cases. Significant up-regulation of cofilin 1 mRNA was associated with stages T0 - T1 and T2; up-regulation seen at stage T3 was not significant compared with control tissue. Fascin mRNA levels were significantly reduced at all three tumour stages (T0 - T1, T2 and T3) compared with control tissue. In conclusion, some components of the actin cytoskeletal system might hold significant potential as targets in future cancer therapies.
MeSH terms
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Actinin / genetics
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Actinin / metabolism
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Adenocarcinoma / genetics
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Adenocarcinoma / metabolism
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Adenocarcinoma / secondary*
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Adolescent
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Adult
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Aged
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Aged, 80 and over
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Breast / metabolism
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Breast / pathology
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Breast Neoplasms / genetics
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Breast Neoplasms / metabolism
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Breast Neoplasms / pathology*
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cofilin 1 / genetics
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Cofilin 1 / metabolism*
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Female
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Fibroadenoma / genetics
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Fibroadenoma / metabolism
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Fibroadenoma / pathology*
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Gene Expression
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Humans
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Mastectomy
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Microfilament Proteins / genetics
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Microfilament Proteins / metabolism*
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Middle Aged
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Peptide Elongation Factor 1 / genetics
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Peptide Elongation Factor 1 / metabolism
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RNA, Messenger / metabolism
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Young Adult
Substances
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ACTN4 protein, human
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CFL1 protein, human
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Carrier Proteins
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Cofilin 1
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EEF1A2 protein, human
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FSCN1 protein, human
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Microfilament Proteins
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Peptide Elongation Factor 1
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RNA, Messenger
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Actinin