Expression of the reversion-inducing cysteine-rich protein with Kazal motifs and matrix metalloproteinase-14 in neuroblastoma and the role in tumour metastasis

Int J Exp Pathol. 2010 Aug;91(4):368-73. doi: 10.1111/j.1365-2613.2010.00724.x. Epub 2010 Jun 24.

Abstract

Neuroblastoma is the most common malignant tumour in infancy; the reversion-inducing cysteine-rich protein with Kazal motifs gene (RECK) is a tumour suppressor gene. Previous studies show that RECK inhibits tumour invasion and metastasis through negative regulation of the matrix metalloproteinase (MMP)-2, MMP-9 and MMP-14. Therefore, we wanted to detect the expression of RECK and MMP-14 in neuroblastomas to assess the correlation between the expression levels of these proteins, and to investigate the roles in the metastasis and development of the tumour. PV-6000 immunohistochemistry method was used to detect the expression levels of RECK and MMP-14 in 36 samples of neuroblastoma tissue. Samples from paraffin wax-embedded specimens and the complete clinicopathological data of 36 neuroblastoma and 10 ganglioneuroma patients were collected. The rate of expression of the RECK protein in the neuroblastoma was low (16.7%). Furthermore, it reduced with the increase in the invasive depth and distant metastasis (P = 0.015; P < 0.05). The rate of expression of the MMP-14 protein in the neuroblastoma was high (58.3%) and increased with the increase in the extent of invasive depth and distant metastasis (P = 0.002; P < 0.05). The expression of the RECK protein correlated negatively with that of MMP-14 (r = -0.418; P < 0.05). Low levels of the RECK protein are expressed in the neuroblastoma, while the MMP-14 protein is expressed at high levels. The RECK and MMP-14 proteins may serve as markers in the estimation of the extent of metastasis and dissemination of the neuroblastoma.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / analysis*
  • Child
  • Child, Preschool
  • Female
  • GPI-Linked Proteins
  • Ganglioneuroma / enzymology*
  • Ganglioneuroma / pathology
  • Humans
  • Immunohistochemistry
  • Infant
  • Lymphatic Metastasis
  • Male
  • Matrix Metalloproteinase 14 / analysis*
  • Membrane Glycoproteins / analysis*
  • Neoplasm Invasiveness
  • Neoplasm Staging
  • Neuroblastoma / enzymology*
  • Neuroblastoma / secondary
  • Paraffin Embedding

Substances

  • Biomarkers, Tumor
  • GPI-Linked Proteins
  • Membrane Glycoproteins
  • RECK protein, human
  • MMP14 protein, human
  • Matrix Metalloproteinase 14