Three novel molecular forms of biliary glycoprotein deduced from cDNA clones from a human leukocyte library

Biochem Biophys Res Commun. 1991 Apr 30;176(2):578-85. doi: 10.1016/s0006-291x(05)80223-2.

Abstract

Three cDNA clones that encode three novel variants of biliary glycoprotein a (BGPa), a glycoprotein belonging to the CEA gene family, were identified in a human leukocyte cDNA library. The domain structures of the predicted proteins of the three clones W211, W233 and W239 are N-A1-B1-A2, N-A1-B1 and N-A1-B1-C, respectively; they lack the transmembrane and cytoplasmic domains that exist in the four BGP species (BGPa, BGPb, BGPc and BGPd) previously reported. Their sequences from N to B1 or to A2 are virtually identical to those of BGPa-d. Comparison with the genomic sequence for BGPa-d suggested that these three new BGP variants as well as BGPa-d are generated from the same single gene by alternative splicing of RNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD
  • Base Sequence
  • Carcinoembryonic Antigen / genetics
  • Carcinoembryonic Antigen / immunology
  • Cell Adhesion Molecules
  • Cloning, Molecular
  • Codon
  • DNA / chemistry*
  • DNA Probes
  • Genetic Variation
  • Genomic Library
  • Glycoproteins / genetics*
  • Glycoproteins / immunology
  • Humans
  • Leukocytes / metabolism*
  • Molecular Sequence Data
  • RNA Splicing

Substances

  • Antigens, CD
  • CD66 antigens
  • Carcinoembryonic Antigen
  • Cell Adhesion Molecules
  • Codon
  • DNA Probes
  • Glycoproteins
  • DNA

Associated data

  • GENBANK/D90311
  • GENBANK/D90312
  • GENBANK/D90313
  • GENBANK/M62480
  • GENBANK/M62481
  • GENBANK/M62482
  • GENBANK/M69176
  • GENBANK/M69194
  • GENBANK/M72238
  • GENBANK/X53944