HIV-EP2, a new member of the gene family encoding the human immunodeficiency virus type 1 enhancer-binding protein. Comparison with HIV-EP1/PRDII-BF1/MBP-1

J Biol Chem. 1991 May 5;266(13):8590-4.

Abstract

At least two different types of proteins, NF-kappa B/KBF1 and HIV-EP1/PRDII-BF1/MBP-1, which are members of a family of rel oncoproteins and metal-finger proteins, respectively, bind to the human immunodeficiency virus type (HIV-1) enhancer. As a new member of a HIV-EP1 family that is expressed at a high level in T cells, we have isolated cDNA clones of HIV-EP2 by cross-hybridization with HIV-EP1 cDNA. HIV-EP2 protein consists of 1,833 amino acids and has a molecular weight of 211,000. HIV-EP2 protein is highly homologous with HIV-EP1/PRDII-BF1/MBP-1 in three regions. These three regions contain the potential nuclear localization signal followed by a Ser/Thr-rich region, the DNA-binding domain consisting of a metal-finger structure, and a cluster of acidic amino acids. The DNA-binding property of HIV-EP2 was similar to that of HIV-EP1. Northern blot analysis of HIV-EP2 mRNA indicated relatively high expression in the T cell line Molt-4 and in some tumor cell lines. Furthermore, like HIV-EP1, expression of HIV-EP2 mRNA was greatly induced by mitogen and phorbol ester treatment of Jurkat T cells, suggesting that HIV-EP2 acts in HIV production from latently infected T cells.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Blotting, Northern
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Genes, Viral*
  • HIV Enhancer*
  • HIV-1 / genetics*
  • Molecular Sequence Data
  • Multigene Family*
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Transcription Factors

Substances

  • Carrier Proteins
  • DNA-Binding Proteins
  • HIVEP1 protein, human
  • Transcription Factors
  • HIVEP2 protein, human

Associated data

  • GENBANK/M59917
  • GENBANK/M60119
  • GENBANK/M60203
  • GENBANK/M60204
  • GENBANK/M60205
  • GENBANK/M60206
  • GENBANK/M62780
  • GENBANK/M63260
  • GENBANK/M63261
  • GENBANK/M63262